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- W2023017066 abstract "A modified enhanced chemiluminescent assay was assessed for its ability to detect oocysts of Cryptosporidium parvum using either X-ray film or a photomultiplier for imaging. The photomultiplier was able to detect a minimum theoretical number of 6.25 purified oocysts and did not appear to have a problem with the detection of false positives. However, because of the plate format of this system, it is not possible to confirm results by microscopy. When X-ray film was utilised for imaging, false positives were detected on 16% (425) occasions, but as few as two oocysts could be detected by this system. X-ray film was also used in the screening of environmental samples previously assessed by microscopy. Although 23% (313) of the negative samples were considered by BEC to be positive, all those samples which contained oocysts were also found to be positive by this system. Until such time as this technique can be refined by increasing the avidity of the monoclonal antibody, enhanced chemiluminescence might provide a useful method for initial screening of environmental and water samples for Cryptosporidium oocysts, with subsequent confirmation by microscopy of samples considered to be positive." @default.
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- W2023017066 date "1993-06-01" @default.
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- W2023017066 title "Detection of oocysts of Cryptosporidium by enhanced chemiluminescence" @default.
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- W2023017066 doi "https://doi.org/10.1016/0167-7012(93)90059-q" @default.
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