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• W2023066176 endingPage "309" @default.
• W2023066176 startingPage "305" @default.
• W2023066176 abstract "Recrudescent herpes labialis (RHL) is a disease caused by herpes simplex virus (HSV). predominantly type 1 (HSV-1). We have monitored HSV-1 shedding in the oral cavity by polymerase chain reaction (PCR) and enzyme-linked immunosorbent assays (ELISA) using digoxigenin-labeled primers designed to amplify a 278 bp segment of the HSV-1 UL 42 region. Prodromal RHL was confirmed by thermographic imaging in 22 patients. Infectious virus was not detected using tissue culture for virus isolation (0/22). Using PCR and agarose gel electrophoresis. We could detect HSV-1 DNA in 8/22 patients. Using a biotinylated-probe internal to the predicted sequence of the PCR product. HSV-1 DNA was detected in 10/22 of the patients by ELISA. We conclude that HSV-1 DNA is shed into the oral cavity of patients presenting with sub-clinical RHL and that the PCR-EL1SA technique represents a more sensitive method to monitor HSV-1 shedding than conventional tissue culturing or PCR-electrophoresis alone." @default.
• W2023066176 created "2016-06-24" @default.
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• W2023066176 date "1997-08-01" @default.
• W2023066176 modified "2023-09-27" @default.
• W2023066176 title "Detection of herpes simplex virus type 1 shedding in the oral cavity by polymerase chain reaction and enzyme-linked immunosorbent assay at the prodromal stage of recrudescent herpes labialis" @default.
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• W2023066176 doi "https://doi.org/10.1111/j.1600-0714.1997.tb00220.x" @default.
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