FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023084885> ?p ?o ?g. }

• W2023084885 endingPage "2045" @default.
• W2023084885 startingPage "2036" @default.
• W2023084885 abstract "Hydrophobic interactions play an active role in effective complex formation between ferredoxin-NADP+ reductase (FNR) and ferredoxin (Fd) from Anabaena, where an aromatic amino acid residue on the Fd surface (F65) and three hydrophobic residues (L76, L78, and V136) on the reductase surface have been shown to be essential for the efficient electron transfer (ET) reaction between Fd and FNR (Martínez-Júlvez et al. (2001) J. Biol. Chem. 276, 27498−27510). Since in this system flavodoxin (Fld) can efficiently replace Fd in the overall ET process, we have further investigated if such hydrophobic interactions are also critical in complex stabilization and ET in the FNR/Fld association. Different ET behaviors with Fld are observed for some of the mutations made at L76, L78, and V136 of Anabaena FNR. Thus, the ET interaction with Fld is almost completely lost upon introduction of negatively charged side chains at these positions, while more conservative changes in the hydrophobic patch can influence the rates of ET to and from Fld by altering the binding constants and the midpoint redox potentials of the flavin group. Therefore, our results confirm that nonpolar residues in the region close to the FAD group in FNR participate in the establishment of interactions with Fld, which serve to orient the two flavin groups in a manner such that ET is favored. In an attempt to look for the counterpart region of the Fld surface, the effect produced by the replacement of the only two nonpolar residues on the Fld surface, I59 and I92, by a Lys has also been analyzed. The results obtained suggest that these two hydrophobic residues are not critical in the interaction and ET processes with FNR. The reactivity of these I92 and I59 Fld mutants toward the membrane-anchored photosystem I (PSI) complex was also analyzed by laser flash absorption spectroscopy. From these data, significant effects are evident, especially for the I92 position of Fld, both in the association constant for complex formation and in the electron-transfer rate constant in the PSI/Fld system." @default.
• W2023084885 created "2016-06-24" @default.
• W2023084885 creator A5001849114 @default.
• W2023084885 creator A5012627839 @default.
• W2023084885 creator A5019324286 @default.
• W2023084885 creator A5021335979 @default.
• W2023084885 creator A5035547103 @default.
• W2023084885 creator A5038223808 @default.
• W2023084885 creator A5047721604 @default.
• W2023084885 creator A5049125752 @default.
• W2023084885 creator A5050421351 @default.
• W2023084885 creator A5058167768 @default.
• W2023084885 creator A5062143235 @default.
• W2023084885 date "2003-01-23" @default.
• W2023084885 modified "2023-10-18" @default.
• W2023084885 title "Role of Hydrophobic Interactions in the Flavodoxin Mediated Electron Transfer from Photosystem I to Ferredoxin-NADP⁺ Reductase in <i>Anabaena</i> PCC 7119" @default.
• W2023084885 cites W1513441752 @default.
• W2023084885 cites W1566546528 @default.
• W2023084885 cites W1796807472 @default.
• W2023084885 cites W190738413 @default.
• W2023084885 cites W1974907033 @default.
• W2023084885 cites W2027134804 @default.
• W2023084885 cites W2038093030 @default.
• W2023084885 cites W2052707761 @default.
• W2023084885 cites W2057940760 @default.
• W2023084885 cites W2082749740 @default.
• W2023084885 cites W2113684150 @default.
• W2023084885 cites W2152639174 @default.
• W2023084885 cites W2167587785 @default.
• W2023084885 cites W4236861131 @default.
• W2023084885 doi "https://doi.org/10.1021/bi0270541" @default.
• W2023084885 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12590591" @default.
• W2023084885 hasPublicationYear "2003" @default.
• W2023084885 type Work @default.
• W2023084885 sameAs 2023084885 @default.
• W2023084885 citedByCount "30" @default.
• W2023084885 countsByYear W20230848852012 @default.
• W2023084885 countsByYear W20230848852013 @default.
• W2023084885 countsByYear W20230848852014 @default.
• W2023084885 countsByYear W20230848852015 @default.
• W2023084885 countsByYear W20230848852016 @default.
• W2023084885 countsByYear W20230848852018 @default.
• W2023084885 countsByYear W20230848852021 @default.
• W2023084885 countsByYear W20230848852022 @default.
• W2023084885 countsByYear W20230848852023 @default.
• W2023084885 crossrefType "journal-article" @default.
• W2023084885 hasAuthorship W2023084885A5001849114 @default.
• W2023084885 hasAuthorship W2023084885A5012627839 @default.
• W2023084885 hasAuthorship W2023084885A5019324286 @default.
• W2023084885 hasAuthorship W2023084885A5021335979 @default.
• W2023084885 hasAuthorship W2023084885A5035547103 @default.
• W2023084885 hasAuthorship W2023084885A5038223808 @default.
• W2023084885 hasAuthorship W2023084885A5047721604 @default.
• W2023084885 hasAuthorship W2023084885A5049125752 @default.
• W2023084885 hasAuthorship W2023084885A5050421351 @default.
• W2023084885 hasAuthorship W2023084885A5058167768 @default.
• W2023084885 hasAuthorship W2023084885A5062143235 @default.
• W2023084885 hasConcept C107563273 @default.
• W2023084885 hasConcept C123669783 @default.
• W2023084885 hasConcept C134621786 @default.
• W2023084885 hasConcept C134651460 @default.
• W2023084885 hasConcept C154828652 @default.
• W2023084885 hasConcept C178790620 @default.
• W2023084885 hasConcept C181199279 @default.
• W2023084885 hasConcept C183688256 @default.
• W2023084885 hasConcept C185592680 @default.
• W2023084885 hasConcept C2776961318 @default.
• W2023084885 hasConcept C2777530773 @default.
• W2023084885 hasConcept C2777737464 @default.
• W2023084885 hasConcept C2779669040 @default.
• W2023084885 hasConcept C33093398 @default.
• W2023084885 hasConcept C3623737 @default.
• W2023084885 hasConcept C523546767 @default.
• W2023084885 hasConcept C54355233 @default.
• W2023084885 hasConcept C55493867 @default.
• W2023084885 hasConcept C55904794 @default.
• W2023084885 hasConcept C71240020 @default.
• W2023084885 hasConcept C71995715 @default.
• W2023084885 hasConcept C75473681 @default.
• W2023084885 hasConcept C8010536 @default.
• W2023084885 hasConcept C80298142 @default.
• W2023084885 hasConcept C86803240 @default.
• W2023084885 hasConcept C90260826 @default.
• W2023084885 hasConceptScore W2023084885C107563273 @default.
• W2023084885 hasConceptScore W2023084885C123669783 @default.
• W2023084885 hasConceptScore W2023084885C134621786 @default.
• W2023084885 hasConceptScore W2023084885C134651460 @default.
• W2023084885 hasConceptScore W2023084885C154828652 @default.
• W2023084885 hasConceptScore W2023084885C178790620 @default.
• W2023084885 hasConceptScore W2023084885C181199279 @default.
• W2023084885 hasConceptScore W2023084885C183688256 @default.
• W2023084885 hasConceptScore W2023084885C185592680 @default.
• W2023084885 hasConceptScore W2023084885C2776961318 @default.
• W2023084885 hasConceptScore W2023084885C2777530773 @default.
• W2023084885 hasConceptScore W2023084885C2777737464 @default.
• W2023084885 hasConceptScore W2023084885C2779669040 @default.
• W2023084885 hasConceptScore W2023084885C33093398 @default.
• W2023084885 hasConceptScore W2023084885C3623737 @default.

• next