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- W2023100327 abstract "Bacillus subtilis PE-11 cells were immobilized in calcium alginate and used for the production of alkaline protease. The influence of alginate concentration, different cations, concentration of cation, curing time, bead diameter and nutrient strength on alkaline protease production and stability of biocatalyst were investigated. Repeated batch fermentations of immobilized cells in shake flasks were carried out with the optimized parameters such as; 3% alginate, 0.25 M calcium chloride with 1 h curing time, 3.24 mm bead diameter and 0.75% glucose and 0.75% peptone as nutrients. The results indicated that, a good level of enzyme was maintained for a period of about 9 days. The immobilized cells of B. subtilis PE-11 in calcium alginate are more efficient for the production of alkaline protease with repeated batch fermentation." @default.
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- W2023100327 date "2004-07-01" @default.
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- W2023100327 title "Investigations on alkaline protease production with B. subtilis PE-11 immobilized in calcium alginate gel beads" @default.
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- W2023100327 doi "https://doi.org/10.1016/s0032-9592(03)00263-2" @default.
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