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- W2023107716 abstract "To obtain fermentable sugar from agarose, pretreatment of agarose by using acetic acid was conducted for short durations (10–30 min) at low acid concentrations (1–5% (w/v)) and high temperatures (110–130 °C). On testing the pretreated agarose by using an endo-β-agarase I (DagA), an exo-β-agarase II (Aga50D), and neoagarobiose hydrolase (NABH), we observed that the addition of the endo-type agarase did not increase the sugar yield. Use of the crude enzyme of Vibrio sp. EJY3 in combination with Aga50D and NABH including acetic acid pretreatment resulted in a 1.3-fold increase in the final reducing sugar yield (62.8% of theoretical maximum based on galactose and 3,6-anhydrogalactose in the initial agarose), compared to those obtained using Aga50D and NABH only after acetic acid pretreatment. The simultaneous saccharification and fermentation of pretreated agarose yielded ethanol of 37.1% theoretical maximum yield from galactose contained in the pretreated agarose." @default.
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- W2023107716 date "2013-05-01" @default.
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- W2023107716 title "High temperature and low acid pretreatment and agarase treatment of agarose for the production of sugar and ethanol from red seaweed biomass" @default.
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- W2023107716 doi "https://doi.org/10.1016/j.biortech.2013.03.038" @default.
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