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- W2023111834 abstract "Abstract A method is described for the rapid and semi-quantitative assessment of the extent of metal ion binding to microbial cell surfaces. The technique is based on the affinity of such surfaces, which are predominantly anionic (by virtue of carboxyl, phosphate and other exposed groups), for binding cationic dyes, and on the ability of exogenous metal ions in solution to compete with and reverse dye binding. Thus, Janus Green bound to intact cells of Pseudomonas cepacia is readily displaced by one of several metal ions. For Cu, dye displacement is rapid ( 2+ > Cd 2+ > Cu 2+ > Ni 2+ > Co 2+ and, for the transition metals, is in accord with the Irving-Williams series. The method is readily scaled down and allows measurement of metal-induced dye displacement from cells in a single colony from solid medium. The advantages and limitations of the procedure are discussed and its wide applicability in biosorption studies proposed." @default.
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- W2023111834 date "1990-04-01" @default.
- W2023111834 modified "2023-09-27" @default.
- W2023111834 title "Displacement of surface-bound cationic dyes: a method for the rapid and semi-quantitative assay of metal binding to microbial surfaces" @default.
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- W2023111834 doi "https://doi.org/10.1016/0167-7012(90)90009-u" @default.
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