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- W2023118204 abstract "We report that two different micropolystyrene round beads linked to either luminol or lucigenin have been developed. The 1-μm-diameter beads are useful reagents that allow one to perform chemiluminescent (light producing) phagocytic reactions without opsonization. It is known that luminol reacts with peroxidases (e.g., myeloperoxidase) while lucigenin reacts mainly with superoxide anion. Luminol and lucigenin beads produce cellular chemiluminescence (CL) with neutrophils, macrophages, monocytes, and differentiated human promyelocytic leukemic cells (HL60). When myeloperoxidase (MPO) is present luminol beads evoke higher CL from neutrophils, but when little or no MPO is present, lucigenin beads produce higher amounts of light. Lucigenin beads appear to be active inside these cells where unbound lucigenin does not enter freely." @default.
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- W2023118204 date "1990-04-01" @default.
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- W2023118204 title "Luminol- or lucigenin-coated micropolystyrene beads, a single reagent to study opsonin-independent phagocytosis by cellular chemiluminescence: Reaction with human neutrophils, monocytes, and differentiated HL60 cells" @default.
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- W2023118204 doi "https://doi.org/10.1016/0026-265x(90)90119-p" @default.
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