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- W2023132083 abstract "Introduction: A nanobiosensor based on fluorescence resonance energy transfer (FRET) for detection of Helicobacter using two specified oligonucleotides conjugated with quantum dot and tamra was fabricated. Luminescent semiconductor quantum dots have been intensely studied due to their unique optical properties. In particular, semiconductor QDs are very attractive as biological labels because of their small size, emission tunability, superior photostability and longer photoluminescence (PL) decay times in comparison to conventional organic dyes. Methods: The water-soluble CdSe/ZnS QDs were prepared. Subsequently, two specific oligonucleotides for Helicobacter were attached to the QDs and tamra to form functional QDand tamra-oligonucleotide conjugates. Result: Along with the addition of DNA targets to a solution containing QDand tamra-oligonucleotide conjugates, hybridization was effectively formed. The resulting assembly brings the tamra fluorophore as an acceptor and the QDs as a donor into the proximity of each other, leading to fluorescence emission transfer from the acceptor by means of FRET phenomenon on illumination of the donor. In the absence of target DNA, the detection probes were not ligated and no tamra emission was produced due to the lack of FRET. Conclusion: The feasibility of the proposed method was also demonstrated in the detection of synthetic 210-mer nucleotide derived from Helicobacter with a sensitivity of 4.0 nM. So here, we conclude that the proposed homogeneously DNA detection method which is simple, rapid and efficient due to the elimination of the washing and separation steps, may be useful in fabrication of a high sensitive nanobiosensor for detection of Helicobacter pylori." @default.
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- W2023132083 date "2011-09-01" @default.
- W2023132083 modified "2023-09-25" @default.
- W2023132083 title "A new and highly sensitive nanobiosensor for detection of Helicobacter based on fluorescence resonance energy transfer" @default.
- W2023132083 doi "https://doi.org/10.1016/j.clinbiochem.2011.08.982" @default.
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