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- W2023135002 abstract "Restriction–modification controller proteins play an essential role in regulating the temporal expression of restriction–modification genes. The controller protein C.Csp231I represents a new class of controller proteins. The gene was sublconed to allow overexpression in Escherichia coli. The protein was purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to 2.0 Å resolution and belonged to space group P21. An electrophoretic mobility-shift assay provided evidence of strong binding of C.Csp231I to a sequence located upstream of the csp231IC start codon." @default.
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- W2023135002 date "2009-08-22" @default.
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- W2023135002 title "Overexpression, purification and preliminary X-ray diffraction analysis of the controller protein C.<i>Csp</i>231I from<i>Citrobacter</i>sp. RFL231" @default.
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- W2023135002 doi "https://doi.org/10.1107/s1744309109028681" @default.
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