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• W2023137150 abstract "Abstract Objective To evaluate primary cultures from human conjunctiva supplemented with fetal bovine serum, autologous serum, and platelet-rich autologous serum, over human amniotic membrane and lens anterior capsules. Methods One-hundred and forty-eight human conjunctiva explants were cultured in CnT50 ® supplemented with 1, 2.5, 5 and 10% fetal bovine serum, autologous serum and platelet-rich autologous serum. Conjunctival samples were incubated at 37 °C, 5% CO 2 and 95% HR, for 3 weeks. Results The typical phenotype corresponding to conjunctival epithelial cells was present in all primary cultures. Conjunctival cultures had MUC5AC-positive secretory cells, K19-positive conjunctival cells, and MUC4-positive non-secretory conjunctival cells, but were not corneal phenotype (cytokeratin K3-negative) and fibroblasts (CD90-negative). Conclusions Conjunctiva epithelial progenitor cells were preserved in all cultures; thus, a cell culture in CnT50 ® supplemented with 1–5% autologous serum over human amniotic membrane can provide better information of epithelial cell differentiation for the conjunctival surface reconstruction." @default.
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• W2023137150 date "2014-01-01" @default.
• W2023137150 modified "2023-10-17" @default.
• W2023137150 title "Characterization of epithelial primary culture from human conjunctiva" @default.
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• W2023137150 doi "https://doi.org/10.1016/j.oftale.2014.03.002" @default.
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