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• W2023138072 endingPage "95" @default.
• W2023138072 startingPage "85" @default.
• W2023138072 abstract "Ferric nitrilotriacetate (Fe3+-NTA) catalyzed hydrogen peroxide-derived production of hydroxyl radicals, which are known to cause DNA damage. In the present work, Fe3+-NTA plus hydrogen peroxide-induced single-strand DNA breaks and repair of the DNA damage were studied in vitro by monitoring DNA damage- and DNA repair-dependent conformational changes of pUC18 plasmid DNA. Single-strand DNA breaks were induced in the pUC18 DNA by Fe3+-NTA plus hydrogen peroxide in a dose-dependent fashion. Induction of the DNA damage was inhibited by deferoxamine mesylate (an iron chelator) and by hydroxyl radical scavengers such as dimethyl sulfoxide (DMSO), d-mannitol and ethanol indicating that the DNA damage was caused by hydroxyl radicals with were generated by reaction of Fe3+-NTA with hydrogen peroxide. The oxygen radical-induced single-strand DNA breaks were repaired partly (more than 50%) by incubating the damaged DNA at 37° C for 3 h with a partially purified preparation of APEX nuclease (a multifunctional DNA repair enzyme), DNA polymerase β, four deoxyribonucleoside triphosphates, T4 DNA ligase and ATP. Analyses of the partially purified preparation of APEX nuclease revealed that a 45-kDa protein as well as APEX nuclease in the preparation were involved in the repair of the single-strand DNA breaks. APEX nuclease was suggested to initiate the repair by removing 3′ termini blocked by the nucleotide fragments and also by incising the 5′ side of AP sites. The 45-kDa protein was suggested to be required for removal of the 5′ tags such as 5′-terminal deoxyribose phosphate residues produced by the action of APEX nuclease on AP sites." @default.
• W2023138072 created "2016-06-24" @default.
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• W2023138072 date "1995-09-01" @default.
• W2023138072 modified "2023-09-24" @default.
• W2023138072 title "Oxygen radical-induced single-strand DNA breaks and repair of the damage in a cell-free system" @default.
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• W2023138072 doi "https://doi.org/10.1016/0921-8777(95)00012-9" @default.
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• W2023138072 hasPublicationYear "1995" @default.
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