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- W2023143334 abstract "Control RNA for RT-PCR applications was amplified by nucleic acid sequence based amplification (NASBA) using the NucliSens® Basic Kit. This method was used to construct positive control RNA for enterovirus, insulin, and G-protein RT-PCR, and for interferon-α real-time RT-PCR. The primers were designed to amplify identical RNA from RNA templates, which differs from the usual NASBA procedure, where opposite strand RNA is amplified from the target. This “inverse NASBA” method is easy to use and it does not require any expensive special equipment. The amplification reaction is done using a water bath and detection of amplified product by agarose gel electrophoresis. Generated RNA fragments were 195–714 bases long, of positive polarity and the amount of RNA was sufficient for thousands of RT-PCR reactions depending on the sensitivity of the RT-PCR." @default.
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- W2023143334 date "2006-01-01" @default.
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- W2023143334 title "Detection and genetic characterisation of circulating measles virus in Ireland" @default.
- W2023143334 doi "https://doi.org/10.1016/s1386-6532(06)80882-6" @default.
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