FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023153977> ?p ?o ?g. }

• W2023153977 abstract "Background: Alpha-1-microglobulin (A1M), a small lipocalin protein found in plasma and tissues, has been identified as a heme and radical scavenger that may participate in the mitigation of toxicities caused by degradation of hemoglobin. The objective of this work was to investigate heme interactions with A1M in vitro using various analytical techniques and to optimize analytical methodology suitable for rapid evaluation of the ligand binding properties of recombinant A1M versions. Methods: To examine heme binding properties of A1M we utilized UV/Vis absorption spectroscopy, visible circular dichroism (CD), catalase-like activity, migration shift electrophoresis, and surface plasmon resonance (SPR), which was specifically developed for the assessment of His-tagged A1M. Results: The results of this study confirm that A1M is a heme binding protein that can accommodate heme at more than one binding site and/or in coordination with different amino acid residues depending upon heme concentration and ligand-to-protein molar ratio. UV/Vis titration of A1M with heme revealed an unusually large bathochromic shift, up to 38 nm, observed for heme binding to a primary binding site. UV/Vis spectroscopy, visible CD and catalase-like activity suggested that heme is accommodated inside His-tagged (tgA1M) and tagless A1M (ntA1M) in a rather similar fashion although the His-tag is very likely involved into coordination with iron of the heme molecule. SPR data indicated kinetic rate constants and equilibrium binding constants with KD values in a uM range. Conclusions: This study provided experimental evidence of the A1M heme binding properties by aid of different techniques and suggested an analytical methodology for a rapid evaluation of ligand-binding properties of recombinant A1M versions, also suitable for other His-tagged proteins." @default.
• W2023153977 created "2016-06-24" @default.
• W2023153977 creator A5011786490 @default.
• W2023153977 creator A5019279142 @default.
• W2023153977 creator A5027623966 @default.
• W2023153977 creator A5041902578 @default.
• W2023153977 creator A5066753320 @default.
• W2023153977 creator A5078640285 @default.
• W2023153977 date "2014-12-04" @default.
• W2023153977 modified "2023-10-16" @default.
• W2023153977 title "Characterization of heme binding to recombinant α1-microglobulin" @default.
• W2023153977 cites W1494194121 @default.
• W2023153977 cites W1573445951 @default.
• W2023153977 cites W1906149389 @default.
• W2023153977 cites W1968125027 @default.
• W2023153977 cites W1975150243 @default.
• W2023153977 cites W1980493860 @default.
• W2023153977 cites W1984887661 @default.
• W2023153977 cites W1988165974 @default.
• W2023153977 cites W1994708463 @default.
• W2023153977 cites W2004647042 @default.
• W2023153977 cites W2005986251 @default.
• W2023153977 cites W2006754144 @default.
• W2023153977 cites W2007719947 @default.
• W2023153977 cites W2008935666 @default.
• W2023153977 cites W2015041458 @default.
• W2023153977 cites W2016847790 @default.
• W2023153977 cites W2018419826 @default.
• W2023153977 cites W2020207911 @default.
• W2023153977 cites W2026733551 @default.
• W2023153977 cites W2028514569 @default.
• W2023153977 cites W2029010155 @default.
• W2023153977 cites W2038592173 @default.
• W2023153977 cites W2039868172 @default.
• W2023153977 cites W2047445353 @default.
• W2023153977 cites W2051933574 @default.
• W2023153977 cites W2051993645 @default.
• W2023153977 cites W2054802708 @default.
• W2023153977 cites W2055710073 @default.
• W2023153977 cites W2056556235 @default.
• W2023153977 cites W2084925214 @default.
• W2023153977 cites W2086259971 @default.
• W2023153977 cites W2090456498 @default.
• W2023153977 cites W2092222846 @default.
• W2023153977 cites W2092934024 @default.
• W2023153977 cites W2095477436 @default.
• W2023153977 cites W2096936713 @default.
• W2023153977 cites W2097102287 @default.
• W2023153977 cites W2109607534 @default.
• W2023153977 cites W2115170303 @default.
• W2023153977 cites W2136937133 @default.
• W2023153977 cites W2147219401 @default.
• W2023153977 cites W2149396121 @default.
• W2023153977 cites W2164793815 @default.
• W2023153977 cites W2169952241 @default.
• W2023153977 cites W2335120678 @default.
• W2023153977 cites W42228917 @default.
• W2023153977 cites W817522 @default.
• W2023153977 doi "https://doi.org/10.3389/fphys.2014.00465" @default.
• W2023153977 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4255499" @default.
• W2023153977 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25538624" @default.
• W2023153977 hasPublicationYear "2014" @default.
• W2023153977 type Work @default.
• W2023153977 sameAs 2023153977 @default.
• W2023153977 citedByCount "31" @default.
• W2023153977 countsByYear W20231539772015 @default.
• W2023153977 countsByYear W20231539772016 @default.
• W2023153977 countsByYear W20231539772017 @default.
• W2023153977 countsByYear W20231539772018 @default.
• W2023153977 countsByYear W20231539772019 @default.
• W2023153977 countsByYear W20231539772020 @default.
• W2023153977 countsByYear W20231539772021 @default.
• W2023153977 countsByYear W20231539772022 @default.
• W2023153977 crossrefType "journal-article" @default.
• W2023153977 hasAuthorship W2023153977A5011786490 @default.
• W2023153977 hasAuthorship W2023153977A5019279142 @default.
• W2023153977 hasAuthorship W2023153977A5027623966 @default.
• W2023153977 hasAuthorship W2023153977A5041902578 @default.
• W2023153977 hasAuthorship W2023153977A5066753320 @default.
• W2023153977 hasAuthorship W2023153977A5078640285 @default.
• W2023153977 hasBestOaLocation W20231539771 @default.
• W2023153977 hasConcept C116569031 @default.
• W2023153977 hasConcept C12554922 @default.
• W2023153977 hasConcept C133571119 @default.
• W2023153977 hasConcept C170493617 @default.
• W2023153977 hasConcept C181199279 @default.
• W2023153977 hasConcept C185592680 @default.
• W2023153977 hasConcept C20705724 @default.
• W2023153977 hasConcept C2776217839 @default.
• W2023153977 hasConcept C55493867 @default.
• W2023153977 hasConcept C86803240 @default.
• W2023153977 hasConceptScore W2023153977C116569031 @default.
• W2023153977 hasConceptScore W2023153977C12554922 @default.
• W2023153977 hasConceptScore W2023153977C133571119 @default.
• W2023153977 hasConceptScore W2023153977C170493617 @default.
• W2023153977 hasConceptScore W2023153977C181199279 @default.
• W2023153977 hasConceptScore W2023153977C185592680 @default.
• W2023153977 hasConceptScore W2023153977C20705724 @default.
• W2023153977 hasConceptScore W2023153977C2776217839 @default.
• W2023153977 hasConceptScore W2023153977C55493867 @default.

• next