FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023162377> ?p ?o ?g. }

• W2023162377 endingPage "137" @default.
• W2023162377 startingPage "124" @default.
• W2023162377 abstract "It has been extremely difficult to elicit broadly cross-reactive neutralizing antibodies (Nabs) against human immunodeficiency virus type 1 (HIV-1). In this study, we compared the immunogenic properties of the wild-type and variable loop-deleted HIV-1 envelope glycoproteins. Mice were immunized with recombinant vaccinia viruses expressing either the wild-type or the variable loop-deleted (V1-2, V3, V4, and V1-3) HIV-1(DH12) gp160s. The animals were subsequently boosted with respective recombinant gp120s. All envelope constructs elicited similar levels of gp120-binding antibodies when analyzed by enzyme-linked immunosorbent assay (ELISA). However, the highest neutralizing activity was observed in sera from animals immunized with the wild-type envelope protein, followed by those immunized with DeltaV4 and DeltaV1-2. No neutralizing activity was detected in sera from animals immunized with DeltaV3 or DeltaV1-3. To identify immunogenic epitopes, ELISA was performed with overlapping 15-mer peptides that cover the entire length of gp120. For the wild-type gp120, the immunogenic epitopes mapped primarily to the variable loops V1-2 and to the conserved regions C1 and C5. When they were plotted onto known coordinates of gp120 core crystal structure, the epitopes in the conserved regions mapped predominantly to the inner domain of the protein. By immunizing with variable loop-deleted envelopes, the immune responses could be redirected to other regions of the protein. However, the newly targeted epitopes were neither on the exposed surface of the protein nor on the receptor binding regions. Interestingly, the removal of the V3 loop resulted in loss of immunoreactivity for both V3 and V1/V2 loops, suggesting structural interaction between the two regions. Our results suggest that obtaining broadly reactive Nabs may not be achieved simply by deleting the variable loops of gp120. However, the observation that the immune responses could be redirected by altering the protein composition might allow us to explore alternative strategies for modifying the antigenic properties of HIV-1 envelope glycoprotein." @default.
• W2023162377 created "2016-06-24" @default.
• W2023162377 creator A5008042637 @default.
• W2023162377 creator A5047252953 @default.
• W2023162377 creator A5071352515 @default.
• W2023162377 creator A5072696015 @default.
• W2023162377 date "2003-01-01" @default.
• W2023162377 modified "2023-09-27" @default.
• W2023162377 title "Immunogenicity and Ability of Variable Loop-Deleted Human Immunodeficiency Virus Type 1 Envelope Glycoproteins to Elicit Neutralizing Antibodies" @default.
• W2023162377 cites W1505517942 @default.
• W2023162377 cites W1628484047 @default.
• W2023162377 cites W1655043483 @default.
• W2023162377 cites W1842798465 @default.
• W2023162377 cites W1879790343 @default.
• W2023162377 cites W1895363198 @default.
• W2023162377 cites W1911432365 @default.
• W2023162377 cites W1929811921 @default.
• W2023162377 cites W1933773138 @default.
• W2023162377 cites W1942872605 @default.
• W2023162377 cites W1948737260 @default.
• W2023162377 cites W1971333195 @default.
• W2023162377 cites W1972385943 @default.
• W2023162377 cites W1978512485 @default.
• W2023162377 cites W1981440980 @default.
• W2023162377 cites W1983365822 @default.
• W2023162377 cites W1986159600 @default.
• W2023162377 cites W2005411001 @default.
• W2023162377 cites W2007282352 @default.
• W2023162377 cites W2010281083 @default.
• W2023162377 cites W2014713450 @default.
• W2023162377 cites W2018999514 @default.
• W2023162377 cites W2020948366 @default.
• W2023162377 cites W2030646603 @default.
• W2023162377 cites W2030675767 @default.
• W2023162377 cites W2032898755 @default.
• W2023162377 cites W2036018115 @default.
• W2023162377 cites W2036206152 @default.
• W2023162377 cites W2039861490 @default.
• W2023162377 cites W2039943899 @default.
• W2023162377 cites W2042256559 @default.
• W2023162377 cites W2042476299 @default.
• W2023162377 cites W2048574795 @default.
• W2023162377 cites W2060830625 @default.
• W2023162377 cites W2062291376 @default.
• W2023162377 cites W2063808707 @default.
• W2023162377 cites W2068490834 @default.
• W2023162377 cites W2070129786 @default.
• W2023162377 cites W2079683200 @default.
• W2023162377 cites W2082678300 @default.
• W2023162377 cites W2085577978 @default.
• W2023162377 cites W2085662163 @default.
• W2023162377 cites W2086725873 @default.
• W2023162377 cites W2088403913 @default.
• W2023162377 cites W2090763940 @default.
• W2023162377 cites W2098781795 @default.
• W2023162377 cites W2099381548 @default.
• W2023162377 cites W2099749788 @default.
• W2023162377 cites W2100860018 @default.
• W2023162377 cites W2105250572 @default.
• W2023162377 cites W2105679023 @default.
• W2023162377 cites W2111105264 @default.
• W2023162377 cites W2113216940 @default.
• W2023162377 cites W2117230039 @default.
• W2023162377 cites W2124195126 @default.
• W2023162377 cites W2124896598 @default.
• W2023162377 cites W2127682720 @default.
• W2023162377 cites W2128055354 @default.
• W2023162377 cites W2137966586 @default.
• W2023162377 cites W2152975649 @default.
• W2023162377 cites W2156557297 @default.
• W2023162377 cites W2159872710 @default.
• W2023162377 cites W2162071888 @default.
• W2023162377 cites W2178148957 @default.
• W2023162377 doi "https://doi.org/10.1006/viro.2002.1727" @default.
• W2023162377 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12504547" @default.
• W2023162377 hasPublicationYear "2003" @default.
• W2023162377 type Work @default.
• W2023162377 sameAs 2023162377 @default.
• W2023162377 citedByCount "57" @default.
• W2023162377 countsByYear W20231623772012 @default.
• W2023162377 countsByYear W20231623772014 @default.
• W2023162377 countsByYear W20231623772016 @default.
• W2023162377 countsByYear W20231623772019 @default.
• W2023162377 countsByYear W20231623772021 @default.
• W2023162377 crossrefType "journal-article" @default.
• W2023162377 hasAuthorship W2023162377A5008042637 @default.
• W2023162377 hasAuthorship W2023162377A5047252953 @default.
• W2023162377 hasAuthorship W2023162377A5071352515 @default.
• W2023162377 hasAuthorship W2023162377A5072696015 @default.
• W2023162377 hasBestOaLocation W20231623771 @default.
• W2023162377 hasConcept C104317684 @default.
• W2023162377 hasConcept C108625454 @default.
• W2023162377 hasConcept C14086860 @default.
• W2023162377 hasConcept C153911025 @default.
• W2023162377 hasConcept C159047783 @default.
• W2023162377 hasConcept C159654299 @default.
• W2023162377 hasConcept C195616568 @default.
• W2023162377 hasConcept C203014093 @default.

• next