FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023163378> ?p ?o ?g. }

• W2023163378 endingPage "425" @default.
• W2023163378 startingPage "420" @default.
• W2023163378 abstract "Lysozyme acts as an important defensive factor in innate immunity due to its well-recognized bacteriolytic activity. Here we describe the production and performance of human lysozyme-like 6 (LYZL6), a novel human c-type lysozyme homolog. A synthetic codon-optimized cDNA encoding the intact amino acid sequence of LYZL6 was cloned and expressed in Pichia pastoris SMD1168. Bioactive LYZL6 was successfully produced as a single major secreted protein with a molecular weight of 15 kDa, and exhibited bacteriolytic activity against Micrococcus lysodeikticus. The expression conditions were optimized, and the highest expression level of LYZL6 occurred when the recombinant strain was induced with 1.5% methanol under pH 4.5 at 24°C for 96 h. When high cell density fermentation of the recombinant P. pastoris was performed using a fed-batch strategy for totally 125 h in a 30 L fermenter, the dry cell weight and the extracellular lysozyme activity were increased to 116.3 g/L and 2340 U/mL, respectively. The LYZL6 protein concentration was 331 mg/L of fermentation supernatant, and the specific activity of LYZL6 towards M. lysodeikticus was 7069 U/mg. Therefore, we proved that LYZL6 is an antibacterial protein, suggesting a potential application of LYZL6 as an antimicrobial agent, and Pichia expression system for LYZL6 was successful and industrially promising." @default.
• W2023163378 created "2016-06-24" @default.
• W2023163378 creator A5018073672 @default.
• W2023163378 creator A5024177859 @default.
• W2023163378 creator A5033530469 @default.
• W2023163378 creator A5086438190 @default.
• W2023163378 date "2014-10-01" @default.
• W2023163378 modified "2023-10-18" @default.
• W2023163378 title "Production of LYZL6, a novel human c-type lysozyme, in recombinant Pichia pastoris employing high cell density fed-batch fermentation" @default.
• W2023163378 cites W1546022138 @default.
• W2023163378 cites W1595540384 @default.
• W2023163378 cites W1781268596 @default.
• W2023163378 cites W1977261492 @default.
• W2023163378 cites W1980344530 @default.
• W2023163378 cites W1983886574 @default.
• W2023163378 cites W1986198847 @default.
• W2023163378 cites W1988398601 @default.
• W2023163378 cites W1991890196 @default.
• W2023163378 cites W1993039539 @default.
• W2023163378 cites W2001430800 @default.
• W2023163378 cites W2012411223 @default.
• W2023163378 cites W2023349270 @default.
• W2023163378 cites W2028875171 @default.
• W2023163378 cites W2030642888 @default.
• W2023163378 cites W2043646113 @default.
• W2023163378 cites W2046879739 @default.
• W2023163378 cites W2051043527 @default.
• W2023163378 cites W2051403769 @default.
• W2023163378 cites W2063554961 @default.
• W2023163378 cites W2071761024 @default.
• W2023163378 cites W2078431207 @default.
• W2023163378 cites W2081363906 @default.
• W2023163378 cites W2082581989 @default.
• W2023163378 cites W2086806574 @default.
• W2023163378 cites W2100241857 @default.
• W2023163378 cites W2100837269 @default.
• W2023163378 cites W2115688626 @default.
• W2023163378 cites W2115905186 @default.
• W2023163378 cites W2134994535 @default.
• W2023163378 cites W2137108435 @default.
• W2023163378 cites W2159056360 @default.
• W2023163378 cites W2164821085 @default.
• W2023163378 cites W4246533287 @default.
• W2023163378 cites W4248619807 @default.
• W2023163378 cites W4293247451 @default.
• W2023163378 doi "https://doi.org/10.1016/j.jbiosc.2014.03.009" @default.
• W2023163378 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24745549" @default.
• W2023163378 hasPublicationYear "2014" @default.
• W2023163378 type Work @default.
• W2023163378 sameAs 2023163378 @default.
• W2023163378 citedByCount "17" @default.
• W2023163378 countsByYear W20231633782015 @default.
• W2023163378 countsByYear W20231633782016 @default.
• W2023163378 countsByYear W20231633782017 @default.
• W2023163378 countsByYear W20231633782018 @default.
• W2023163378 countsByYear W20231633782019 @default.
• W2023163378 countsByYear W20231633782021 @default.
• W2023163378 countsByYear W20231633782023 @default.
• W2023163378 crossrefType "journal-article" @default.
• W2023163378 hasAuthorship W2023163378A5018073672 @default.
• W2023163378 hasAuthorship W2023163378A5024177859 @default.
• W2023163378 hasAuthorship W2023163378A5033530469 @default.
• W2023163378 hasAuthorship W2023163378A5086438190 @default.
• W2023163378 hasConcept C100544194 @default.
• W2023163378 hasConcept C104317684 @default.
• W2023163378 hasConcept C167431671 @default.
• W2023163378 hasConcept C185592680 @default.
• W2023163378 hasConcept C187882448 @default.
• W2023163378 hasConcept C2776966407 @default.
• W2023163378 hasConcept C2781372317 @default.
• W2023163378 hasConcept C28406088 @default.
• W2023163378 hasConcept C40767141 @default.
• W2023163378 hasConcept C547475151 @default.
• W2023163378 hasConcept C55493867 @default.
• W2023163378 hasConcept C73401843 @default.
• W2023163378 hasConcept C86803240 @default.
• W2023163378 hasConceptScore W2023163378C100544194 @default.
• W2023163378 hasConceptScore W2023163378C104317684 @default.
• W2023163378 hasConceptScore W2023163378C167431671 @default.
• W2023163378 hasConceptScore W2023163378C185592680 @default.
• W2023163378 hasConceptScore W2023163378C187882448 @default.
• W2023163378 hasConceptScore W2023163378C2776966407 @default.
• W2023163378 hasConceptScore W2023163378C2781372317 @default.
• W2023163378 hasConceptScore W2023163378C28406088 @default.
• W2023163378 hasConceptScore W2023163378C40767141 @default.
• W2023163378 hasConceptScore W2023163378C547475151 @default.
• W2023163378 hasConceptScore W2023163378C55493867 @default.
• W2023163378 hasConceptScore W2023163378C73401843 @default.
• W2023163378 hasConceptScore W2023163378C86803240 @default.
• W2023163378 hasIssue "4" @default.
• W2023163378 hasLocation W20231633781 @default.
• W2023163378 hasLocation W20231633782 @default.
• W2023163378 hasOpenAccess W2023163378 @default.
• W2023163378 hasPrimaryLocation W20231633781 @default.
• W2023163378 hasRelatedWork W1976031800 @default.
• W2023163378 hasRelatedWork W2048318784 @default.
• W2023163378 hasRelatedWork W2105971127 @default.
• W2023163378 hasRelatedWork W2358773353 @default.

• next