Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023163708> ?p ?o ?g. }
- W2023163708 endingPage "1831" @default.
- W2023163708 startingPage "1819" @default.
- W2023163708 abstract "Neurodegenerative illnesses are characterized by aberrant metabolism of biometals such as copper (Cu), zinc (Zn) and iron (Fe). However, little is known about the metabolic effects associated with altered metal homeostasis. In this study, we used an in vitro model of altered Cu homeostasis to investigate how Cu regulates cellular protein expression. Human fibroblasts containing a natural deletion mutation of the Menkes (MNK) ATP7A Cu transporter (MNK deleted) were compared to fibroblasts overexpressing ATP7A (MNK transfected). Cultures of MNK-transfected (Low-Cu) cells exhibited 95% less intracellular Cu than MNK-deleted (High-Cu) cells. Comparative proteomic analysis of the two cell-lines was performed using antibody microarrays, and significant differential protein expression was observed between Low-Cu and High-Cu cell-lines. Western blot analysis confirmed the altered protein expression of Ku80, nexilin, L-caldesmon, MAP4, Inhibitor 2 and DNA topoisomerase I. The top 50 altered proteins were analysed using the software program Pathway Studio (Ariadne Genomics) and revealed a significant over-representation of proteins involved in DNA repair and maintenance. Further analysis confirmed that expression of the DNA repair protein Ku80 was dependent on cellular Cu homeostasis and that Low-Cu levels in fibroblasts resulted in elevated susceptibility to DNA oxidation." @default.
- W2023163708 created "2016-06-24" @default.
- W2023163708 creator A5002372957 @default.
- W2023163708 creator A5006373060 @default.
- W2023163708 creator A5006623638 @default.
- W2023163708 creator A5012093503 @default.
- W2023163708 creator A5026838566 @default.
- W2023163708 creator A5033772486 @default.
- W2023163708 creator A5033891416 @default.
- W2023163708 creator A5038374630 @default.
- W2023163708 creator A5045915090 @default.
- W2023163708 creator A5047385288 @default.
- W2023163708 creator A5054892819 @default.
- W2023163708 creator A5061389893 @default.
- W2023163708 creator A5074285807 @default.
- W2023163708 creator A5075346937 @default.
- W2023163708 creator A5082082058 @default.
- W2023163708 date "2008-05-01" @default.
- W2023163708 modified "2023-10-06" @default.
- W2023163708 title "Investigating copper-regulated protein expression in Menkes fibroblasts using antibody microarrays" @default.
- W2023163708 cites W1502029262 @default.
- W2023163708 cites W1538748480 @default.
- W2023163708 cites W1546952499 @default.
- W2023163708 cites W1707143272 @default.
- W2023163708 cites W1976908176 @default.
- W2023163708 cites W1977145510 @default.
- W2023163708 cites W2001483897 @default.
- W2023163708 cites W2001953177 @default.
- W2023163708 cites W2002946614 @default.
- W2023163708 cites W2004175706 @default.
- W2023163708 cites W2006909664 @default.
- W2023163708 cites W2012054697 @default.
- W2023163708 cites W2013444425 @default.
- W2023163708 cites W2014093336 @default.
- W2023163708 cites W2024166316 @default.
- W2023163708 cites W2024586422 @default.
- W2023163708 cites W2028346440 @default.
- W2023163708 cites W2032699581 @default.
- W2023163708 cites W2033692581 @default.
- W2023163708 cites W2037550429 @default.
- W2023163708 cites W2039517040 @default.
- W2023163708 cites W2042675162 @default.
- W2023163708 cites W2043488589 @default.
- W2023163708 cites W2043503470 @default.
- W2023163708 cites W2044627978 @default.
- W2023163708 cites W2052987925 @default.
- W2023163708 cites W2060036094 @default.
- W2023163708 cites W2072550971 @default.
- W2023163708 cites W2078374046 @default.
- W2023163708 cites W2079190863 @default.
- W2023163708 cites W2079758822 @default.
- W2023163708 cites W2081098333 @default.
- W2023163708 cites W2083500052 @default.
- W2023163708 cites W2101163066 @default.
- W2023163708 cites W2110280725 @default.
- W2023163708 cites W2128389187 @default.
- W2023163708 cites W2142415307 @default.
- W2023163708 cites W2147701483 @default.
- W2023163708 cites W2154852310 @default.
- W2023163708 cites W2157935345 @default.
- W2023163708 cites W2159895255 @default.
- W2023163708 cites W2199166367 @default.
- W2023163708 cites W65097477 @default.
- W2023163708 doi "https://doi.org/10.1002/pmic.200700895" @default.
- W2023163708 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18384105" @default.
- W2023163708 hasPublicationYear "2008" @default.
- W2023163708 type Work @default.
- W2023163708 sameAs 2023163708 @default.
- W2023163708 citedByCount "7" @default.
- W2023163708 countsByYear W20231637082012 @default.
- W2023163708 countsByYear W20231637082016 @default.
- W2023163708 crossrefType "journal-article" @default.
- W2023163708 hasAuthorship W2023163708A5002372957 @default.
- W2023163708 hasAuthorship W2023163708A5006373060 @default.
- W2023163708 hasAuthorship W2023163708A5006623638 @default.
- W2023163708 hasAuthorship W2023163708A5012093503 @default.
- W2023163708 hasAuthorship W2023163708A5026838566 @default.
- W2023163708 hasAuthorship W2023163708A5033772486 @default.
- W2023163708 hasAuthorship W2023163708A5033891416 @default.
- W2023163708 hasAuthorship W2023163708A5038374630 @default.
- W2023163708 hasAuthorship W2023163708A5045915090 @default.
- W2023163708 hasAuthorship W2023163708A5047385288 @default.
- W2023163708 hasAuthorship W2023163708A5054892819 @default.
- W2023163708 hasAuthorship W2023163708A5061389893 @default.
- W2023163708 hasAuthorship W2023163708A5074285807 @default.
- W2023163708 hasAuthorship W2023163708A5075346937 @default.
- W2023163708 hasAuthorship W2023163708A5082082058 @default.
- W2023163708 hasConcept C104317684 @default.
- W2023163708 hasConcept C134935766 @default.
- W2023163708 hasConcept C149011108 @default.
- W2023163708 hasConcept C150194340 @default.
- W2023163708 hasConcept C153911025 @default.
- W2023163708 hasConcept C178790620 @default.
- W2023163708 hasConcept C185592680 @default.
- W2023163708 hasConcept C2776415932 @default.
- W2023163708 hasConcept C2777581567 @default.
- W2023163708 hasConcept C2779736553 @default.
- W2023163708 hasConcept C2991729501 @default.