FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023163726> ?p ?o ?g. }

• W2023163726 endingPage "e30809" @default.
• W2023163726 startingPage "e30809" @default.
• W2023163726 abstract "Cap analysis of gene expression (CAGE) is a 5' sequence tag technology to globally determine transcriptional starting sites in the genome and their expression levels and has most recently been adapted to the HeliScope single molecule sequencer. Despite significant simplifications in the CAGE protocol, it has until now been a labour intensive protocol.In this study we set out to adapt the protocol to a robotic workflow, which would increase throughput and reduce handling. The automated CAGE cDNA preparation system we present here can prepare 96 'HeliScope ready' CAGE cDNA libraries in 8 days, as opposed to 6 weeks by a manual operator.We compare the results obtained using the same RNA in manual libraries and across multiple automation batches to assess reproducibility.We show that the sequencing was highly reproducible and comparable to manual libraries with an 8 fold increase in productivity. The automated CAGE cDNA preparation system can prepare 96 CAGE sequencing samples simultaneously. Finally we discuss how the system could be used for CAGE on Illumina/SOLiD platforms, RNA-seq and full-length cDNA generation." @default.
• W2023163726 created "2016-06-24" @default.
• W2023163726 creator A5019747814 @default.
• W2023163726 creator A5032097008 @default.
• W2023163726 creator A5035595400 @default.
• W2023163726 creator A5038594088 @default.
• W2023163726 creator A5049377448 @default.
• W2023163726 creator A5051284054 @default.
• W2023163726 creator A5057612742 @default.
• W2023163726 creator A5061403923 @default.
• W2023163726 creator A5065938896 @default.
• W2023163726 creator A5072131150 @default.
• W2023163726 creator A5075290044 @default.
• W2023163726 date "2012-01-30" @default.
• W2023163726 modified "2023-09-26" @default.
• W2023163726 title "Automated Workflow for Preparation of cDNA for Cap Analysis of Gene Expression on a Single Molecule Sequencer" @default.
• W2023163726 cites W1560200430 @default.
• W2023163726 cites W162187343 @default.
• W2023163726 cites W1921457844 @default.
• W2023163726 cites W1979273476 @default.
• W2023163726 cites W1979926492 @default.
• W2023163726 cites W1982575478 @default.
• W2023163726 cites W1984708728 @default.
• W2023163726 cites W2007771322 @default.
• W2023163726 cites W2015416439 @default.
• W2023163726 cites W2017840637 @default.
• W2023163726 cites W2031932327 @default.
• W2023163726 cites W2047285578 @default.
• W2023163726 cites W2047765648 @default.
• W2023163726 cites W2052576920 @default.
• W2023163726 cites W2057692691 @default.
• W2023163726 cites W2059699217 @default.
• W2023163726 cites W2061747833 @default.
• W2023163726 cites W2082777953 @default.
• W2023163726 cites W2097117811 @default.
• W2023163726 cites W2105776281 @default.
• W2023163726 cites W2114703458 @default.
• W2023163726 cites W2117706405 @default.
• W2023163726 cites W2121211805 @default.
• W2023163726 cites W2127310510 @default.
• W2023163726 cites W2127994696 @default.
• W2023163726 cites W2145483178 @default.
• W2023163726 cites W2154431984 @default.
• W2023163726 cites W2156165856 @default.
• W2023163726 cites W2159091439 @default.
• W2023163726 cites W2162304711 @default.
• W2023163726 cites W2171111703 @default.
• W2023163726 cites W2247766769 @default.
• W2023163726 cites W4233902414 @default.
• W2023163726 cites W90820373 @default.
• W2023163726 doi "https://doi.org/10.1371/journal.pone.0030809" @default.
• W2023163726 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3268765" @default.
• W2023163726 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22303458" @default.
• W2023163726 hasPublicationYear "2012" @default.
• W2023163726 type Work @default.
• W2023163726 sameAs 2023163726 @default.
• W2023163726 citedByCount "22" @default.
• W2023163726 countsByYear W20231637262013 @default.
• W2023163726 countsByYear W20231637262014 @default.
• W2023163726 countsByYear W20231637262015 @default.
• W2023163726 countsByYear W20231637262017 @default.
• W2023163726 countsByYear W20231637262019 @default.
• W2023163726 countsByYear W20231637262020 @default.
• W2023163726 countsByYear W20231637262021 @default.
• W2023163726 countsByYear W20231637262022 @default.
• W2023163726 countsByYear W20231637262023 @default.
• W2023163726 crossrefType "journal-article" @default.
• W2023163726 hasAuthorship W2023163726A5019747814 @default.
• W2023163726 hasAuthorship W2023163726A5032097008 @default.
• W2023163726 hasAuthorship W2023163726A5035595400 @default.
• W2023163726 hasAuthorship W2023163726A5038594088 @default.
• W2023163726 hasAuthorship W2023163726A5049377448 @default.
• W2023163726 hasAuthorship W2023163726A5051284054 @default.
• W2023163726 hasAuthorship W2023163726A5057612742 @default.
• W2023163726 hasAuthorship W2023163726A5061403923 @default.
• W2023163726 hasAuthorship W2023163726A5065938896 @default.
• W2023163726 hasAuthorship W2023163726A5072131150 @default.
• W2023163726 hasAuthorship W2023163726A5075290044 @default.
• W2023163726 hasBestOaLocation W20231637261 @default.
• W2023163726 hasConcept C104317684 @default.
• W2023163726 hasConcept C177212765 @default.
• W2023163726 hasConcept C187882448 @default.
• W2023163726 hasConcept C189482833 @default.
• W2023163726 hasConcept C41008148 @default.
• W2023163726 hasConcept C51679486 @default.
• W2023163726 hasConcept C54355233 @default.
• W2023163726 hasConcept C61053724 @default.
• W2023163726 hasConcept C70712619 @default.
• W2023163726 hasConcept C70721500 @default.
• W2023163726 hasConcept C77088390 @default.
• W2023163726 hasConcept C86803240 @default.
• W2023163726 hasConceptScore W2023163726C104317684 @default.
• W2023163726 hasConceptScore W2023163726C177212765 @default.
• W2023163726 hasConceptScore W2023163726C187882448 @default.
• W2023163726 hasConceptScore W2023163726C189482833 @default.
• W2023163726 hasConceptScore W2023163726C41008148 @default.
• W2023163726 hasConceptScore W2023163726C51679486 @default.
• W2023163726 hasConceptScore W2023163726C54355233 @default.

• next