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- W2023169044 abstract "Growth of Tetrahymena pyriformis W was used to assess the relative nutritive value (RNV) of 40 foodstuffs. Growth of the organism was estimated by ammonia-nitrogen (NH3-N) accumulation, media optical density (OD) and the reduction of triphenyl-tetrazolium chloride (TTC) for triphenyl-formazan. The latter approach was evaluated both on a linear and on a logarithmic scale. All methods used to determine RNC were compared to the microscope cell-counting procedure before and after adjustment by linear regression equations. When compared to cell counts, the measurement of NH3-N accumulation resulted in the lowest mean difference, followed by the measurements of TTC reduction on a logarithmic scale, OD and then TTC reduction on a linear scale. Mean deviations from cell count values were reduced for all methods after regression equations were used to fit experimental values to the same range as the cell count values. It was concluded that cell counts should be used to calculate RNV and that NH3-N or OD determinations could increase the efficiency of the RNV procedure for preliminary sample screening." @default.
- W2023169044 created "2016-06-24" @default.
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- W2023169044 date "1978-08-01" @default.
- W2023169044 modified "2023-09-25" @default.
- W2023169044 title "Comparisons of methods used for estimating the growth ofTetrahymena pyriformis W" @default.
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- W2023169044 doi "https://doi.org/10.1002/jsfa.2740290808" @default.
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