FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023170633> ?p ?o ?g. }

• W2023170633 endingPage "129" @default.
• W2023170633 startingPage "124" @default.
• W2023170633 abstract "PhaA from Ralstonia eutropha (RePhaA) is the first enzyme in the polyhydroxyalbutyrate (PHB) biosynthetic pathway and catalyzes the condensation of two molecules of acetyl-CoA to acetoacetyl-CoA. To investigate the molecular mechanism underlying PHB biosynthesis, we determined the crystal structures of the RePhaA protein in apo- and CoA-bound forms. The RePhaA structure adopts the type II biosynthetic thiolase fold forming a tetramer by means of dimerization of two dimers. The crystal structure of RePhaA in complex with CoA revealed that the enzyme contained a unique Phe219 residue, resulting that the ADP moiety binds in somewhat different position compared with that bound in other thiolase enzymes. Our study provides structural insight into the substrate specificity of RePhaA. Results indicate the presence of a small pocket near the Cys88 covalent catalytic residue leading to the possibility of the enzyme to accommodate acetyl-CoA as a sole substrate instead of larger acyl-CoA molecules such as propionyl-CoA. Furthermore, the roles of key residues involved in substrate binding and enzyme catalysis were confirmed by site-directed mutagenesis." @default.
• W2023170633 created "2016-06-24" @default.
• W2023170633 creator A5035243568 @default.
• W2023170633 creator A5091385586 @default.
• W2023170633 date "2014-09-01" @default.
• W2023170633 modified "2023-09-26" @default.
• W2023170633 title "Crystal structure and biochemical characterization of PhaA from Ralstonia eutropha, a polyhydroxyalkanoate-producing bacterium" @default.
• W2023170633 cites W1539796472 @default.
• W2023170633 cites W1944728106 @default.
• W2023170633 cites W1966094758 @default.
• W2023170633 cites W1968817040 @default.
• W2023170633 cites W1979780102 @default.
• W2023170633 cites W1983806484 @default.
• W2023170633 cites W1988398404 @default.
• W2023170633 cites W1995017064 @default.
• W2023170633 cites W2003941318 @default.
• W2023170633 cites W2007186013 @default.
• W2023170633 cites W2011462935 @default.
• W2023170633 cites W2014694459 @default.
• W2023170633 cites W2033809555 @default.
• W2023170633 cites W2038840577 @default.
• W2023170633 cites W2041196825 @default.
• W2023170633 cites W2046018478 @default.
• W2023170633 cites W2052593247 @default.
• W2023170633 cites W2053484261 @default.
• W2023170633 cites W2058360649 @default.
• W2023170633 cites W2065880240 @default.
• W2023170633 cites W2068295003 @default.
• W2023170633 cites W2070939592 @default.
• W2023170633 cites W2081559448 @default.
• W2023170633 cites W2091124319 @default.
• W2023170633 cites W2100448885 @default.
• W2023170633 cites W2103345065 @default.
• W2023170633 cites W2125645956 @default.
• W2023170633 cites W2144081223 @default.
• W2023170633 cites W2146080841 @default.
• W2023170633 cites W2152211203 @default.
• W2023170633 cites W4234022669 @default.
• W2023170633 cites W4242557011 @default.
• W2023170633 doi "https://doi.org/10.1016/j.bbrc.2014.08.074" @default.
• W2023170633 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25152395" @default.
• W2023170633 hasPublicationYear "2014" @default.
• W2023170633 type Work @default.
• W2023170633 sameAs 2023170633 @default.
• W2023170633 citedByCount "17" @default.
• W2023170633 countsByYear W20231706332015 @default.
• W2023170633 countsByYear W20231706332016 @default.
• W2023170633 countsByYear W20231706332017 @default.
• W2023170633 countsByYear W20231706332018 @default.
• W2023170633 countsByYear W20231706332019 @default.
• W2023170633 countsByYear W20231706332021 @default.
• W2023170633 countsByYear W20231706332023 @default.
• W2023170633 crossrefType "journal-article" @default.
• W2023170633 hasAuthorship W2023170633A5035243568 @default.
• W2023170633 hasAuthorship W2023170633A5091385586 @default.
• W2023170633 hasConcept C181199279 @default.
• W2023170633 hasConcept C185592680 @default.
• W2023170633 hasConcept C2776317432 @default.
• W2023170633 hasConcept C2776568683 @default.
• W2023170633 hasConcept C2778886173 @default.
• W2023170633 hasConcept C2780637011 @default.
• W2023170633 hasConcept C2780734198 @default.
• W2023170633 hasConcept C2781031557 @default.
• W2023170633 hasConcept C2781338088 @default.
• W2023170633 hasConcept C41183919 @default.
• W2023170633 hasConcept C523546767 @default.
• W2023170633 hasConcept C54355233 @default.
• W2023170633 hasConcept C553450214 @default.
• W2023170633 hasConcept C55493867 @default.
• W2023170633 hasConcept C71240020 @default.
• W2023170633 hasConcept C73447357 @default.
• W2023170633 hasConcept C86803240 @default.
• W2023170633 hasConceptScore W2023170633C181199279 @default.
• W2023170633 hasConceptScore W2023170633C185592680 @default.
• W2023170633 hasConceptScore W2023170633C2776317432 @default.
• W2023170633 hasConceptScore W2023170633C2776568683 @default.
• W2023170633 hasConceptScore W2023170633C2778886173 @default.
• W2023170633 hasConceptScore W2023170633C2780637011 @default.
• W2023170633 hasConceptScore W2023170633C2780734198 @default.
• W2023170633 hasConceptScore W2023170633C2781031557 @default.
• W2023170633 hasConceptScore W2023170633C2781338088 @default.
• W2023170633 hasConceptScore W2023170633C41183919 @default.
• W2023170633 hasConceptScore W2023170633C523546767 @default.
• W2023170633 hasConceptScore W2023170633C54355233 @default.
• W2023170633 hasConceptScore W2023170633C553450214 @default.
• W2023170633 hasConceptScore W2023170633C55493867 @default.
• W2023170633 hasConceptScore W2023170633C71240020 @default.
• W2023170633 hasConceptScore W2023170633C73447357 @default.
• W2023170633 hasConceptScore W2023170633C86803240 @default.
• W2023170633 hasFunder F4320322120 @default.
• W2023170633 hasFunder F4320322349 @default.
• W2023170633 hasIssue "1" @default.
• W2023170633 hasLocation W20231706331 @default.
• W2023170633 hasLocation W20231706332 @default.
• W2023170633 hasOpenAccess W2023170633 @default.
• W2023170633 hasPrimaryLocation W20231706331 @default.
• W2023170633 hasRelatedWork W1599448530 @default.
• W2023170633 hasRelatedWork W1925427717 @default.

• next