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- W2023171087 abstract "Carbon isotope effect studies were undertaken with the wild-type pyridoxal 5‘-phosphate (PLP)-dependent enzyme ornithine decarboxylase (ODC) from Trypanosoma brucei and with several active site mutants of the enzyme. For the decarboxylation of the optimal substrate, l-ornithine, by wild-type ODC, the observed carbon isotope effect (k12/k13) is 1.033 at pH 7.3. In comparison to the expected intrinsic isotope effect (k12/k13 = 1.06) for decarboxylation, this value suggests that both the rate of decarboxylation and the rate of Schiff base interchange with l-ornithine are partially rate-limiting for the reaction steps up to decarboxylation. In contrast, with the alternate substrate l-Lys, which shows lower catalytic efficiency, the carbon isotope effect increased to 1.063, demonstrating that decarboxylation has become the rate-limiting step. For the mutant enzymes, E274A ODC and C360A ODC, with l-ornithine as substrate the carbon isotope effect also approaches the intrinsic limit. Glu-274 was previously demonstrated to play a direct role in carbanion stabilization, and thus the large carbon isotope effect (k12/k13 = 1.055) is consistent with an impaired rate of decarboxylation compared to wild-type ODC. In contrast, for K69A ODC, the isotope effect is almost entirely suppressed, suggesting that Schiff-base formation (which now must occur from enzyme-bound PLP, rather than from an enzyme-bound PLP−Schiff base) has become rate-determining." @default.
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- W2023171087 date "1998-09-26" @default.
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- W2023171087 title "Carbon-13 Isotope Effect Studies of <i>Trypanosoma brucei</i> Ornithine Decarboxylase" @default.
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- W2023171087 doi "https://doi.org/10.1021/bi981154i" @default.
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