FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023180769> ?p ?o ?g. }

• W2023180769 endingPage "40818" @default.
• W2023180769 startingPage "40809" @default.
• W2023180769 abstract "Nucleoside 5'-O-phosphorothioates are formed in vivo as primary products of hydrolysis of oligo(nucleoside phosphorothioate)s (PS-oligos) that are applied as antisense therapeutic molecules. The biodistribution of PS-oligos and their pharmacokinetics have been widely reported, but little is known about their subsequent decay inside the organism. We suggest that the enzyme responsible for nucleoside 5'-O-monophosphorothioate ((d)NMPS) metabolism could be histidine triad nucleotide-binding protein 1 (Hint-1), a phosphoramidase belonging to the histidine triad (HIT) superfamily that is present in all forms of life. An additional, but usually ignored, activity of Hint-1 is its ability to catalyze the conversion of adenosine 5'-O-monophosphorothioate (AMPS) to 5'-O-monophosphate (AMP). By mutagenetic and biochemical studies, we defined the active site of Hint-1 and the kinetic parameters of the desulfuration reaction (P-S bond cleavage). Additionally, crystallographic analysis (resolution from 1.08 to 1.37 Å) of three engineered cysteine mutants showed the high similarity of their structures, which were not very different from the structure of WT Hint-1. Moreover, we found that not only AMPS but also other ribonucleoside and 2'-deoxyribonucleoside phosphorothioates are desulfurated by Hint-1 at the following relative rates: GMPS > AMPS > dGMPS ≥ CMPS > UMPS > dAMPS ≫ dCMPS > TMPS, and during the reaction, hydrogen sulfide, which is thought to be the third gaseous mediator, was released." @default.
• W2023180769 created "2016-06-24" @default.
• W2023180769 creator A5026229478 @default.
• W2023180769 creator A5032596330 @default.
• W2023180769 creator A5050770101 @default.
• W2023180769 creator A5065760652 @default.
• W2023180769 creator A5086928632 @default.
• W2023180769 date "2010-12-01" @default.
• W2023180769 modified "2023-09-27" @default.
• W2023180769 title "Histidine Triad Nucleotide-binding Protein 1 (HINT-1) Phosphoramidase Transforms Nucleoside 5′-O-Phosphorothioates to Nucleoside 5′-O-Phosphates" @default.
• W2023180769 cites W1531013072 @default.
• W2023180769 cites W1539796472 @default.
• W2023180769 cites W1572550482 @default.
• W2023180769 cites W1973763289 @default.
• W2023180769 cites W1974923961 @default.
• W2023180769 cites W1984201743 @default.
• W2023180769 cites W1986191025 @default.
• W2023180769 cites W1996451919 @default.
• W2023180769 cites W2001641653 @default.
• W2023180769 cites W2002207630 @default.
• W2023180769 cites W2002593242 @default.
• W2023180769 cites W2011968242 @default.
• W2023180769 cites W2015407072 @default.
• W2023180769 cites W2016315684 @default.
• W2023180769 cites W2017901238 @default.
• W2023180769 cites W2023042597 @default.
• W2023180769 cites W2026036133 @default.
• W2023180769 cites W2029748625 @default.
• W2023180769 cites W2038840577 @default.
• W2023180769 cites W2045174016 @default.
• W2023180769 cites W2052569438 @default.
• W2023180769 cites W2054673335 @default.
• W2023180769 cites W2057078208 @default.
• W2023180769 cites W2057259362 @default.
• W2023180769 cites W2059380859 @default.
• W2023180769 cites W2062989669 @default.
• W2023180769 cites W2067401769 @default.
• W2023180769 cites W2079884831 @default.
• W2023180769 cites W2081912151 @default.
• W2023180769 cites W2097493124 @default.
• W2023180769 cites W2124026197 @default.
• W2023180769 cites W2129827813 @default.
• W2023180769 cites W2136567909 @default.
• W2023180769 cites W2138599948 @default.
• W2023180769 cites W2158534713 @default.
• W2023180769 cites W2159495147 @default.
• W2023180769 cites W2165611390 @default.
• W2023180769 cites W2169764921 @default.
• W2023180769 cites W2325127218 @default.
• W2023180769 cites W2949360401 @default.
• W2023180769 cites W2951470777 @default.
• W2023180769 cites W3023454987 @default.
• W2023180769 cites W4210675630 @default.
• W2023180769 cites W4253924196 @default.
• W2023180769 doi "https://doi.org/10.1074/jbc.m110.162065" @default.
• W2023180769 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3003382" @default.
• W2023180769 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20940308" @default.
• W2023180769 hasPublicationYear "2010" @default.
• W2023180769 type Work @default.
• W2023180769 sameAs 2023180769 @default.
• W2023180769 citedByCount "35" @default.
• W2023180769 countsByYear W20231807692012 @default.
• W2023180769 countsByYear W20231807692013 @default.
• W2023180769 countsByYear W20231807692014 @default.
• W2023180769 countsByYear W20231807692015 @default.
• W2023180769 countsByYear W20231807692016 @default.
• W2023180769 countsByYear W20231807692017 @default.
• W2023180769 countsByYear W20231807692018 @default.
• W2023180769 countsByYear W20231807692019 @default.
• W2023180769 countsByYear W20231807692020 @default.
• W2023180769 countsByYear W20231807692021 @default.
• W2023180769 countsByYear W20231807692022 @default.
• W2023180769 countsByYear W20231807692023 @default.
• W2023180769 crossrefType "journal-article" @default.
• W2023180769 hasAuthorship W2023180769A5026229478 @default.
• W2023180769 hasAuthorship W2023180769A5032596330 @default.
• W2023180769 hasAuthorship W2023180769A5050770101 @default.
• W2023180769 hasAuthorship W2023180769A5065760652 @default.
• W2023180769 hasAuthorship W2023180769A5086928632 @default.
• W2023180769 hasBestOaLocation W20231807691 @default.
• W2023180769 hasConcept C104317684 @default.
• W2023180769 hasConcept C129312508 @default.
• W2023180769 hasConcept C181199279 @default.
• W2023180769 hasConcept C185592680 @default.
• W2023180769 hasConcept C2776476023 @default.
• W2023180769 hasConcept C2776543447 @default.
• W2023180769 hasConcept C2777610669 @default.
• W2023180769 hasConcept C2778460671 @default.
• W2023180769 hasConcept C2779201268 @default.
• W2023180769 hasConcept C2780636463 @default.
• W2023180769 hasConcept C512185932 @default.
• W2023180769 hasConcept C552990157 @default.
• W2023180769 hasConcept C55493867 @default.
• W2023180769 hasConcept C71240020 @default.
• W2023180769 hasConcept C71615608 @default.
• W2023180769 hasConceptScore W2023180769C104317684 @default.
• W2023180769 hasConceptScore W2023180769C129312508 @default.
• W2023180769 hasConceptScore W2023180769C181199279 @default.

• next