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• W2023185789 abstract "The biocompatibility of metallic implant surfaces is governed in large part by the interfacial kinetics associated with metal release and protein binding. The kinetics of metal release from, and protein binding to, cobalt- and titanium-based implant alloys in human serum were investigated by (1). measuring the temporal release of Cr and Ti into serum from Co-Cr-Mo (ASTM F-75) and Ti implant alloys (Ti-6Al-4V: ASTM F136, and commercially pure Ti, cpTi: ASTM F67), respectively; (2). examining the composition of human serum proteins adsorbed onto the surfaces of Co- and Ti-based implant alloys; and (3). identifying the serum proteins associated with the binding of soluble Cr and Ti degradation products. Analysis of metal dissolution kinetics found that Cr was released from Co-based implant alloy at an order of magnitude higher than Ti was released from Ti-based implant alloys. Serum became saturated with soluble CR and Ti at levels as high as 3250 ng/mL Ti from cpTi; 3750 ng/mL Ti from Ti-6Al-4V; and 35400 ng/mL Cr from Co-Cr-Mo degradation. The observation that human serum binds more released metal from Co-based alloy dissolution was consistent with the observed differences in biofilm composition between the two alloys, where additional serum protein(s) of approximately approximately 140 (kDa) molecular weight were detected on Co-based implant alloy surfaces. However, both Cr and Ti released from Co- and Ti-based alloys exhibited a bimodal binding pattern to both low molecular weight serum protein(s) (<32 kDa), and to higher molecular weight protein(s) in the 180-250 kDa range. Identification of metal alloy-dependent biofilm compositions and dissolution products provides the basis for understanding the bioavailability and bioreactivity of these implant alloys and their degradation products." @default.
• W2023185789 created "2016-06-24" @default.
• W2023185789 creator A5057383926 @default.
• W2023185789 creator A5079465450 @default.
• W2023185789 creator A5083160859 @default.
• W2023185789 date "2003-04-25" @default.
• W2023185789 modified "2023-10-15" @default.
• W2023185789 title "Interfacial kinetics of titanium- and cobalt-based implant alloys in human serum: Metal release and biofilm formation" @default.
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• W2023185789 doi "https://doi.org/10.1002/jbm.a.10429" @default.
• W2023185789 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12746877" @default.
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