FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023189220> ?p ?o ?g. }

• W2023189220 endingPage "6935" @default.
• W2023189220 startingPage "6931" @default.
• W2023189220 abstract "A human keratinocyte cDNA expression library in bacteriophage lambda gt11 was screened with the purified IgG fraction of serum from a patient with epidermolysis bullosa acquisita, which had a high titer of anti-type VII collagen antibodies. Screening of approximately 3 x 10(5) plaques identified 8 positive clones, the largest one (K-131) being approximately 1.9 kilobases in size. Dideoxynucleotide sequencing of K-131 indicated that it consisted of 1875 base pairs and contained an open reading frame coding for a putative N-terminal noncollagenous domain of 439 amino acids and a collagenous C-terminal segment of 186 amino acids. The collagenous domain was characterized by repeating Gly-Xaa-Yaa sequences that were interrupted in several positions by insertions or deletions of 1-3 amino acids. The deduced amino acid sequence also revealed a peptide segment that had a high degree of identity with a published type VII collagen protein sequence. Northern hybridization of the K-131 cDNA with human epidermal keratinocyte and skin fibroblast RNA revealed an mRNA of approximately 8.5 kilobases. The fusion protein produced by the K-131 cDNA, when incubated with epidermolysis bullosa acquisita serum, bound to antibodies that reacted in Western blots with type VII collagen. The genomic location of the type VII collagen gene (COL7A1) was determined by chromosomal in situ hybridization with the K-131 cDNA. The results mapped the COL7A1 to the locus 3p21. The cDNA clones characterized in this study will be valuable for understanding the protein structure and gene expression of type VII collagen present in anchoring fibrils and its aberrations in the dystrophic forms of heritable epidermolysis bullosa." @default.
• W2023189220 created "2016-06-24" @default.
• W2023189220 creator A5002666266 @default.
• W2023189220 creator A5007855326 @default.
• W2023189220 creator A5032533037 @default.
• W2023189220 creator A5033178471 @default.
• W2023189220 creator A5043264431 @default.
• W2023189220 creator A5056787953 @default.
• W2023189220 creator A5064365750 @default.
• W2023189220 creator A5068354250 @default.
• W2023189220 creator A5085853201 @default.
• W2023189220 date "1991-08-15" @default.
• W2023189220 modified "2023-10-18" @default.
• W2023189220 title "Human type VII collagen: cDNA cloning and chromosomal mapping of the gene." @default.
• W2023189220 cites W1510836818 @default.
• W2023189220 cites W1512432174 @default.
• W2023189220 cites W1520609171 @default.
• W2023189220 cites W1971103686 @default.
• W2023189220 cites W1977660251 @default.
• W2023189220 cites W1985909825 @default.
• W2023189220 cites W1987568997 @default.
• W2023189220 cites W1999947662 @default.
• W2023189220 cites W2000401321 @default.
• W2023189220 cites W2012847227 @default.
• W2023189220 cites W2015292449 @default.
• W2023189220 cites W2029556279 @default.
• W2023189220 cites W2032826590 @default.
• W2023189220 cites W2036730964 @default.
• W2023189220 cites W2038826250 @default.
• W2023189220 cites W2041168586 @default.
• W2023189220 cites W2043705636 @default.
• W2023189220 cites W2049814304 @default.
• W2023189220 cites W2050151051 @default.
• W2023189220 cites W2056357607 @default.
• W2023189220 cites W2060333964 @default.
• W2023189220 cites W2080496169 @default.
• W2023189220 cites W2119518116 @default.
• W2023189220 cites W2138270253 @default.
• W2023189220 cites W2142275262 @default.
• W2023189220 cites W2146059271 @default.
• W2023189220 cites W2169943386 @default.
• W2023189220 cites W2313977109 @default.
• W2023189220 cites W2476542314 @default.
• W2023189220 cites W980313740 @default.
• W2023189220 doi "https://doi.org/10.1073/pnas.88.16.6931" @default.
• W2023189220 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/52207" @default.
• W2023189220 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1871109" @default.
• W2023189220 hasPublicationYear "1991" @default.
• W2023189220 type Work @default.
• W2023189220 sameAs 2023189220 @default.
• W2023189220 citedByCount "188" @default.
• W2023189220 countsByYear W20231892202012 @default.
• W2023189220 countsByYear W20231892202013 @default.
• W2023189220 countsByYear W20231892202014 @default.
• W2023189220 countsByYear W20231892202015 @default.
• W2023189220 countsByYear W20231892202016 @default.
• W2023189220 countsByYear W20231892202017 @default.
• W2023189220 countsByYear W20231892202018 @default.
• W2023189220 countsByYear W20231892202019 @default.
• W2023189220 countsByYear W20231892202021 @default.
• W2023189220 countsByYear W20231892202022 @default.
• W2023189220 countsByYear W20231892202023 @default.
• W2023189220 crossrefType "journal-article" @default.
• W2023189220 hasAuthorship W2023189220A5002666266 @default.
• W2023189220 hasAuthorship W2023189220A5007855326 @default.
• W2023189220 hasAuthorship W2023189220A5032533037 @default.
• W2023189220 hasAuthorship W2023189220A5033178471 @default.
• W2023189220 hasAuthorship W2023189220A5043264431 @default.
• W2023189220 hasAuthorship W2023189220A5056787953 @default.
• W2023189220 hasAuthorship W2023189220A5064365750 @default.
• W2023189220 hasAuthorship W2023189220A5068354250 @default.
• W2023189220 hasAuthorship W2023189220A5085853201 @default.
• W2023189220 hasBestOaLocation W20231892201 @default.
• W2023189220 hasConcept C104317684 @default.
• W2023189220 hasConcept C153911025 @default.
• W2023189220 hasConcept C159654299 @default.
• W2023189220 hasConcept C167625842 @default.
• W2023189220 hasConcept C187882448 @default.
• W2023189220 hasConcept C189482833 @default.
• W2023189220 hasConcept C2779252107 @default.
• W2023189220 hasConcept C2779305546 @default.
• W2023189220 hasConcept C515207424 @default.
• W2023189220 hasConcept C54355233 @default.
• W2023189220 hasConcept C84597430 @default.
• W2023189220 hasConcept C86803240 @default.
• W2023189220 hasConceptScore W2023189220C104317684 @default.
• W2023189220 hasConceptScore W2023189220C153911025 @default.
• W2023189220 hasConceptScore W2023189220C159654299 @default.
• W2023189220 hasConceptScore W2023189220C167625842 @default.
• W2023189220 hasConceptScore W2023189220C187882448 @default.
• W2023189220 hasConceptScore W2023189220C189482833 @default.
• W2023189220 hasConceptScore W2023189220C2779252107 @default.
• W2023189220 hasConceptScore W2023189220C2779305546 @default.
• W2023189220 hasConceptScore W2023189220C515207424 @default.
• W2023189220 hasConceptScore W2023189220C54355233 @default.
• W2023189220 hasConceptScore W2023189220C84597430 @default.
• W2023189220 hasConceptScore W2023189220C86803240 @default.
• W2023189220 hasIssue "16" @default.

• next