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- W2023190078 abstract "(1) Fractionation of the plant enzyme proteins obtained from Squash rind juice was carried out by partial precipitation with ammonium sulfate, from which it was completely revealed that the plant L-glutamic acid decarboxylase preparation as reported in many papers may consist of α-ketoglutaric-γ-aminobutyric transaminase and L-glutamic acid decarboxylase. (2) Further stereochemical evidence of L-glutamic acid was obtained by paper partition chromatography and by paper electrophoresis, and the L-glutamic acid formed by this transaminase was proved to be of the L-form configuration. (3) The preparation containing transaminase obtained here was tested for its activity of L-glutamic acid formation from α-ketoglutaric acid and ammonium sulfate, and always gave negative results. The expence of this research was partly defrayed by a grant in acid from the Ajinomoto Co., Inc., to which the authors' thanks are due. The authors also wish to thank Messrs. T. Miki, N. Tsukada, M. Matsumoto of this laboratory for their generous assistance." @default.
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- W2023190078 date "1958-01-01" @default.
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- W2023190078 title "Formation of L-Glutamic Acid from γ-Aminobutyric Acid by Plant Enzyme" @default.
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- W2023190078 doi "https://doi.org/10.1271/bbb1924.22.341" @default.
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