FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023194635> ?p ?o ?g. }

• W2023194635 endingPage "5816" @default.
• W2023194635 startingPage "5800" @default.
• W2023194635 abstract "Several snake venom secreted phospholipases A2 (sPLA2s) including OS2 exert a variety of pharmacological effects ranging from central neurotoxicity to anti-HIV activity by mechanisms that are not yet fully understood. To conclusively address the role of enzymatic activity and map the key structural elements of OS2 responsible for its pharmacological properties, we have prepared single point OS2 mutants at the catalytic site and large chimeras between OS2 and OS1, a homologous but nontoxic sPLA2. Most importantly, we found that the enzymatic activity of the active site mutant H48Q is 500-fold lower than that of the wild-type protein, while central neurotoxicity is only 16-fold lower, providing convincing evidence that catalytic activity is at most a minor factor that determines central neurotoxicity. The chimera approach has identified the N-terminal region (residues 1-22) of OS2, but not the central one (residues 58-89), as crucial for both enzymatic activity and pharmacological effects. The C-terminal region of OS2 (residues 102-119) was found to be critical for enzymatic activity, but not for central neurotoxicity and anti-HIV activity, allowing us to further dissociate enzymatic activity and pharmacological effects. Finally, direct binding studies with the C-terminal chimera, which poorly binds to phospholipids while it is still neurotoxic, led to the identification of a subset of brain N-type receptors which may be directly involved in central neurotoxicity." @default.
• W2023194635 created "2016-06-24" @default.
• W2023194635 creator A5003513067 @default.
• W2023194635 creator A5004994403 @default.
• W2023194635 creator A5025358774 @default.
• W2023194635 creator A5032600033 @default.
• W2023194635 creator A5034409028 @default.
• W2023194635 creator A5042606678 @default.
• W2023194635 creator A5048085199 @default.
• W2023194635 creator A5051178994 @default.
• W2023194635 creator A5054562365 @default.
• W2023194635 creator A5055859205 @default.
• W2023194635 creator A5063605857 @default.
• W2023194635 creator A5068045779 @default.
• W2023194635 creator A5073988774 @default.
• W2023194635 creator A5078144972 @default.
• W2023194635 creator A5079027751 @default.
• W2023194635 creator A5082931018 @default.
• W2023194635 date "2006-04-12" @default.
• W2023194635 modified "2023-10-16" @default.
• W2023194635 title "Neurotoxicity and Other Pharmacological Activities of the Snake Venom Phospholipase A₂OS₂: The N-Terminal Region Is More Important Than Enzymatic Activity" @default.
• W2023194635 cites W1486402962 @default.
• W2023194635 cites W1499641759 @default.
• W2023194635 cites W1506838377 @default.
• W2023194635 cites W1514236298 @default.
• W2023194635 cites W1554348499 @default.
• W2023194635 cites W1667578667 @default.
• W2023194635 cites W1915678073 @default.
• W2023194635 cites W1969741975 @default.
• W2023194635 cites W1975041299 @default.
• W2023194635 cites W1987660914 @default.
• W2023194635 cites W1988788901 @default.
• W2023194635 cites W1989721734 @default.
• W2023194635 cites W2002225116 @default.
• W2023194635 cites W2004226937 @default.
• W2023194635 cites W2008533226 @default.
• W2023194635 cites W2013654614 @default.
• W2023194635 cites W2014430242 @default.
• W2023194635 cites W2015933480 @default.
• W2023194635 cites W2031632203 @default.
• W2023194635 cites W2040664100 @default.
• W2023194635 cites W2040706747 @default.
• W2023194635 cites W2053878306 @default.
• W2023194635 cites W2056026888 @default.
• W2023194635 cites W2056993318 @default.
• W2023194635 cites W2061798671 @default.
• W2023194635 cites W2062933094 @default.
• W2023194635 cites W2078143666 @default.
• W2023194635 cites W2090879065 @default.
• W2023194635 cites W2132509717 @default.
• W2023194635 cites W2134416921 @default.
• W2023194635 cites W2134571618 @default.
• W2023194635 cites W2140399335 @default.
• W2023194635 cites W2141572434 @default.
• W2023194635 cites W2155362595 @default.
• W2023194635 cites W2168541231 @default.
• W2023194635 cites W4242541818 @default.
• W2023194635 doi "https://doi.org/10.1021/bi060217r" @default.
• W2023194635 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2796912" @default.
• W2023194635 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16669624" @default.
• W2023194635 hasPublicationYear "2006" @default.
• W2023194635 type Work @default.
• W2023194635 sameAs 2023194635 @default.
• W2023194635 citedByCount "62" @default.
• W2023194635 countsByYear W20231946352012 @default.
• W2023194635 countsByYear W20231946352013 @default.
• W2023194635 countsByYear W20231946352014 @default.
• W2023194635 countsByYear W20231946352015 @default.
• W2023194635 countsByYear W20231946352017 @default.
• W2023194635 countsByYear W20231946352018 @default.
• W2023194635 countsByYear W20231946352019 @default.
• W2023194635 countsByYear W20231946352020 @default.
• W2023194635 countsByYear W20231946352021 @default.
• W2023194635 countsByYear W20231946352022 @default.
• W2023194635 countsByYear W20231946352023 @default.
• W2023194635 crossrefType "journal-article" @default.
• W2023194635 hasAuthorship W2023194635A5003513067 @default.
• W2023194635 hasAuthorship W2023194635A5004994403 @default.
• W2023194635 hasAuthorship W2023194635A5025358774 @default.
• W2023194635 hasAuthorship W2023194635A5032600033 @default.
• W2023194635 hasAuthorship W2023194635A5034409028 @default.
• W2023194635 hasAuthorship W2023194635A5042606678 @default.
• W2023194635 hasAuthorship W2023194635A5048085199 @default.
• W2023194635 hasAuthorship W2023194635A5051178994 @default.
• W2023194635 hasAuthorship W2023194635A5054562365 @default.
• W2023194635 hasAuthorship W2023194635A5055859205 @default.
• W2023194635 hasAuthorship W2023194635A5063605857 @default.
• W2023194635 hasAuthorship W2023194635A5068045779 @default.
• W2023194635 hasAuthorship W2023194635A5073988774 @default.
• W2023194635 hasAuthorship W2023194635A5078144972 @default.
• W2023194635 hasAuthorship W2023194635A5079027751 @default.
• W2023194635 hasAuthorship W2023194635A5082931018 @default.
• W2023194635 hasBestOaLocation W20231946354 @default.
• W2023194635 hasConcept C104317684 @default.
• W2023194635 hasConcept C107824862 @default.
• W2023194635 hasConcept C116073593 @default.
• W2023194635 hasConcept C143065580 @default.
• W2023194635 hasConcept C178790620 @default.

• next