Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023194764> ?p ?o ?g. }
- W2023194764 endingPage "680" @default.
- W2023194764 startingPage "673" @default.
- W2023194764 abstract "Pyruvate dehydrogenase complex activity (PDHC) measured by CO2 release isotopic assay has generally been much lower than activity measured by the spec-trophotometric arylamine acetyltransferase assay (ArAT). Decarboxylation of [l-14C]pyruvate was measured in osmotically shocked rat brain cortical mitochondria. Activity is dependent on the concentration of the substrate pyruvate. Activity of 74.6 units ± 12.3 SD (n = 22) was observed at 4 mM pyruvate (1 unit = 1 nmol pyruvate decarboxy-lated/min/mg protein). Activity was dependent on added NAD, CoA, and thiamine pyrophosphate, implying increased mitochondrial permeability after osmotic shock. Freeze/thaw with sonication of the mitochondrial preparation reduced PDHC activity to 11.5 units ± 3.0 SD (n = 4). Oxaloacetate produced a marked stimulation of activity. The optimal assay contained 3 mM oxaloacetate, and without oxaloacetate activity fell to 15.4 units ± 9.9 SD (n = 8). These studies highlight the importance of optimal substrate concentrations in the CO2 release isotopic PDHC method. Higher PDHC activity is found with intact mitochondria and thus activity values should be interpreted in the light of the presence or absence of intact mitochondria in individual preparations." @default.
- W2023194764 created "2016-06-24" @default.
- W2023194764 creator A5001491512 @default.
- W2023194764 creator A5010397370 @default.
- W2023194764 creator A5049009391 @default.
- W2023194764 creator A5073050156 @default.
- W2023194764 creator A5088673881 @default.
- W2023194764 date "1988-03-01" @default.
- W2023194764 modified "2023-09-23" @default.
- W2023194764 title "Pyruvate Dehydrogenase Activity in Osmotically Shocked Rat Brain Mitochondria: Stimulation by Oxaloacetate" @default.
- W2023194764 cites W1254243414 @default.
- W2023194764 cites W1489081871 @default.
- W2023194764 cites W1570859745 @default.
- W2023194764 cites W1595308056 @default.
- W2023194764 cites W1644175625 @default.
- W2023194764 cites W1775749144 @default.
- W2023194764 cites W188366741 @default.
- W2023194764 cites W1968562200 @default.
- W2023194764 cites W1969512045 @default.
- W2023194764 cites W1970431048 @default.
- W2023194764 cites W1976968605 @default.
- W2023194764 cites W1977143304 @default.
- W2023194764 cites W1978319709 @default.
- W2023194764 cites W1980620214 @default.
- W2023194764 cites W1981402692 @default.
- W2023194764 cites W1985522920 @default.
- W2023194764 cites W1990118391 @default.
- W2023194764 cites W1992160777 @default.
- W2023194764 cites W1998373971 @default.
- W2023194764 cites W1998490400 @default.
- W2023194764 cites W2016243001 @default.
- W2023194764 cites W2032234107 @default.
- W2023194764 cites W2041699439 @default.
- W2023194764 cites W2053296812 @default.
- W2023194764 cites W2057714231 @default.
- W2023194764 cites W2089688040 @default.
- W2023194764 cites W2100629926 @default.
- W2023194764 cites W2100936288 @default.
- W2023194764 cites W2113479894 @default.
- W2023194764 cites W2157394103 @default.
- W2023194764 cites W2163302837 @default.
- W2023194764 cites W2168755667 @default.
- W2023194764 cites W2267823124 @default.
- W2023194764 cites W2332150735 @default.
- W2023194764 cites W2332813952 @default.
- W2023194764 cites W2418139113 @default.
- W2023194764 cites W4244133279 @default.
- W2023194764 doi "https://doi.org/10.1111/j.1471-4159.1988.tb02966.x" @default.
- W2023194764 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3339345" @default.
- W2023194764 hasPublicationYear "1988" @default.
- W2023194764 type Work @default.
- W2023194764 sameAs 2023194764 @default.
- W2023194764 citedByCount "6" @default.
- W2023194764 countsByYear W20231947642015 @default.
- W2023194764 countsByYear W20231947642017 @default.
- W2023194764 crossrefType "journal-article" @default.
- W2023194764 hasAuthorship W2023194764A5001491512 @default.
- W2023194764 hasAuthorship W2023194764A5010397370 @default.
- W2023194764 hasAuthorship W2023194764A5049009391 @default.
- W2023194764 hasAuthorship W2023194764A5073050156 @default.
- W2023194764 hasAuthorship W2023194764A5088673881 @default.
- W2023194764 hasConcept C101972281 @default.
- W2023194764 hasConcept C111133373 @default.
- W2023194764 hasConcept C126093377 @default.
- W2023194764 hasConcept C134651460 @default.
- W2023194764 hasConcept C165135838 @default.
- W2023194764 hasConcept C181199279 @default.
- W2023194764 hasConcept C185592680 @default.
- W2023194764 hasConcept C190098323 @default.
- W2023194764 hasConcept C197957613 @default.
- W2023194764 hasConcept C2779123261 @default.
- W2023194764 hasConcept C2780047943 @default.
- W2023194764 hasConcept C28859421 @default.
- W2023194764 hasConcept C55493867 @default.
- W2023194764 hasConcept C62231903 @default.
- W2023194764 hasConcept C86803240 @default.
- W2023194764 hasConcept C88099638 @default.
- W2023194764 hasConceptScore W2023194764C101972281 @default.
- W2023194764 hasConceptScore W2023194764C111133373 @default.
- W2023194764 hasConceptScore W2023194764C126093377 @default.
- W2023194764 hasConceptScore W2023194764C134651460 @default.
- W2023194764 hasConceptScore W2023194764C165135838 @default.
- W2023194764 hasConceptScore W2023194764C181199279 @default.
- W2023194764 hasConceptScore W2023194764C185592680 @default.
- W2023194764 hasConceptScore W2023194764C190098323 @default.
- W2023194764 hasConceptScore W2023194764C197957613 @default.
- W2023194764 hasConceptScore W2023194764C2779123261 @default.
- W2023194764 hasConceptScore W2023194764C2780047943 @default.
- W2023194764 hasConceptScore W2023194764C28859421 @default.
- W2023194764 hasConceptScore W2023194764C55493867 @default.
- W2023194764 hasConceptScore W2023194764C62231903 @default.
- W2023194764 hasConceptScore W2023194764C86803240 @default.
- W2023194764 hasConceptScore W2023194764C88099638 @default.
- W2023194764 hasIssue "3" @default.
- W2023194764 hasLocation W20231947641 @default.
- W2023194764 hasLocation W20231947642 @default.
- W2023194764 hasOpenAccess W2023194764 @default.
- W2023194764 hasPrimaryLocation W20231947641 @default.