FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023197943> ?p ?o ?g. }

• W2023197943 endingPage "1025" @default.
• W2023197943 startingPage "1014" @default.
• W2023197943 abstract "OBJECTIVE: Murine neural stem cells (NSCs) were homografted onto the injured spinal cord (SC) to assess their potential to improve motor behavior, to differentiate as neurons, and to establish synapse-like contacts with the descending axonal paths of the host. In addition, we investigated whether transduced NSCs over-expressing vascular endothelial growth factor might exert any angiogenetic effect in the injured SC. METHODS: NSCs derived from mouse embryos were transduced to express either green fluorescent protein or vascular endothelial growth factor. The cells were engrafted in mice where an extended dorsal funiculotomy had been performed at the T8–T9 level. At intervals from 4 to 12 weeks after grafting, motor behavior was assessed using an open field locomotor scale and footprint analysis. At the same time points, the SC was studied by conventional histology, immunohistochemistry, and fluorescence microscopy. The interactions between the grafted NSCs and descending axonal paths were investigated using anterogradely transported fluorescent axonal tracers. RESULTS: By the 12–week time point, mice engrafted with NSCs significantly improved both their locomotor score on open field test and their base of support on footprint analysis. Histological studies showed that green fluorescent protein-positive NSCs survived as long as 12 weeks after grafting, migrated from the grafting site with a tropism toward the lesion, and either remained undifferentiated or differentiated into the astrocytic phenotype without neuronal or oligodendrocytic differentiation. Interestingly, the NSC-derived astrocytes expressed vimentin, suggesting that these cells differentiated as immature astrocytes. The tips of severed descending axonal paths came adjacent to grafted NSCs without forming synapse-like structures. When genetically engineered to over-express vascular endothelial growth factor, the grafted NSCs significantly increased vessel density in the injured area. CONCLUSION: In the traumatically injured mice SC, NSC grafting improves motor recovery. Although differentiation of engrafted NSCs is restricted exclusively toward the astrocytic phenotype, the NSC-derived astrocytes show features that are typical of the early phase after SC injury when the glial scar is still permissive to regenerating axons. The immature phenotype of the NSC-derived astrocytes suggests that these cells might support neurite outgrowth by the host neurons. Thus, modifying the glial scar with NSCs might enhance axonal regeneration in the injured area. The use of genetically engineered NSCs that express trophic factors appears to be an attractive tool in SC transplantation research." @default.
• W2023197943 created "2016-06-24" @default.
• W2023197943 creator A5008155790 @default.
• W2023197943 creator A5028569565 @default.
• W2023197943 creator A5029877910 @default.
• W2023197943 creator A5035048187 @default.
• W2023197943 creator A5042442375 @default.
• W2023197943 creator A5060090725 @default.
• W2023197943 creator A5065711800 @default.
• W2023197943 creator A5069752420 @default.
• W2023197943 creator A5070104677 @default.
• W2023197943 creator A5078512025 @default.
• W2023197943 creator A5078871025 @default.
• W2023197943 date "2005-11-01" @default.
• W2023197943 modified "2023-10-15" @default.
• W2023197943 title "Homologous Transplantation of Neural Stem Cells to the Injured Spinal Cord of Mice" @default.
• W2023197943 cites W1198538403 @default.
• W2023197943 cites W1487914405 @default.
• W2023197943 cites W1539440045 @default.
• W2023197943 cites W1565233996 @default.
• W2023197943 cites W1566969252 @default.
• W2023197943 cites W1574222606 @default.
• W2023197943 cites W1733539716 @default.
• W2023197943 cites W1859089489 @default.
• W2023197943 cites W1965375858 @default.
• W2023197943 cites W1965933264 @default.
• W2023197943 cites W1967806479 @default.
• W2023197943 cites W1987085702 @default.
• W2023197943 cites W1987804149 @default.
• W2023197943 cites W1988189150 @default.
• W2023197943 cites W1989197038 @default.
• W2023197943 cites W1989242285 @default.
• W2023197943 cites W1996240157 @default.
• W2023197943 cites W2000043284 @default.
• W2023197943 cites W2017599428 @default.
• W2023197943 cites W2018742703 @default.
• W2023197943 cites W2019215467 @default.
• W2023197943 cites W2020155245 @default.
• W2023197943 cites W2025830748 @default.
• W2023197943 cites W2039945427 @default.
• W2023197943 cites W2041418680 @default.
• W2023197943 cites W2045528880 @default.
• W2023197943 cites W2053631104 @default.
• W2023197943 cites W2062841992 @default.
• W2023197943 cites W2064496329 @default.
• W2023197943 cites W2067014198 @default.
• W2023197943 cites W2067198472 @default.
• W2023197943 cites W2070357113 @default.
• W2023197943 cites W2072568062 @default.
• W2023197943 cites W2077398525 @default.
• W2023197943 cites W2077514930 @default.
• W2023197943 cites W2079200499 @default.
• W2023197943 cites W2082451783 @default.
• W2023197943 cites W2092610977 @default.
• W2023197943 cites W2098324605 @default.
• W2023197943 cites W2099498580 @default.
• W2023197943 cites W2107800314 @default.
• W2023197943 cites W2111769538 @default.
• W2023197943 cites W2112408004 @default.
• W2023197943 cites W2117198101 @default.
• W2023197943 cites W2120715240 @default.
• W2023197943 cites W2123048941 @default.
• W2023197943 cites W2123450039 @default.
• W2023197943 cites W2135107819 @default.
• W2023197943 cites W2155626323 @default.
• W2023197943 cites W2155639900 @default.
• W2023197943 cites W2159689992 @default.
• W2023197943 cites W2170645579 @default.
• W2023197943 cites W2331483068 @default.
• W2023197943 doi "https://doi.org/10.1227/01.neu.0000180058.58372.4c" @default.
• W2023197943 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16284571" @default.
• W2023197943 hasPublicationYear "2005" @default.
• W2023197943 type Work @default.
• W2023197943 sameAs 2023197943 @default.
• W2023197943 citedByCount "65" @default.
• W2023197943 countsByYear W20231979432012 @default.
• W2023197943 countsByYear W20231979432013 @default.
• W2023197943 countsByYear W20231979432014 @default.
• W2023197943 countsByYear W20231979432015 @default.
• W2023197943 countsByYear W20231979432016 @default.
• W2023197943 countsByYear W20231979432017 @default.
• W2023197943 countsByYear W20231979432018 @default.
• W2023197943 countsByYear W20231979432019 @default.
• W2023197943 countsByYear W20231979432022 @default.
• W2023197943 crossrefType "journal-article" @default.
• W2023197943 hasAuthorship W2023197943A5008155790 @default.
• W2023197943 hasAuthorship W2023197943A5028569565 @default.
• W2023197943 hasAuthorship W2023197943A5029877910 @default.
• W2023197943 hasAuthorship W2023197943A5035048187 @default.
• W2023197943 hasAuthorship W2023197943A5042442375 @default.
• W2023197943 hasAuthorship W2023197943A5060090725 @default.
• W2023197943 hasAuthorship W2023197943A5065711800 @default.
• W2023197943 hasAuthorship W2023197943A5069752420 @default.
• W2023197943 hasAuthorship W2023197943A5070104677 @default.
• W2023197943 hasAuthorship W2023197943A5078512025 @default.
• W2023197943 hasAuthorship W2023197943A5078871025 @default.
• W2023197943 hasConcept C104317684 @default.
• W2023197943 hasConcept C105702510 @default.

• next