Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023200247> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2023200247 endingPage "283" @default.
- W2023200247 startingPage "271" @default.
- W2023200247 abstract "Gastric mucosal homogenates from hog were fractionated by differential and density gradient centrifugation and free-flow electrophoresis. The two major membrane fractions (FI and FII) thus obtained are distinct both enzymically and in terms of transport reactivity. This heterogenicity extends to their antigenic activity. Purified antibodies which were raised against the K+-ATPase-containing H+ transport fraction FI were of two types: inhibitory and non-inhibitory. Inhibitory antibodies reduced the K+-ATPase activity by approximately 80% and the K+-p-nitro-phenylphosphatase activity by approximately 40% in a concentration-dependent manner, while the small Mg++-dependent component of the enzyme activity was unaffected. Antibodies inhibiting the K+-ATPase also inhibited H+ transport. These antibodies did not cross-react with the other major membrane fraction isolated by free-flow electrophoresis, FII, and gave a single band on rocket immunoelectrophoresis. Antibodies against this FII fraction also did not react with the K+-ATPase and were heterogeneous, giving at least four bands with rocket immunoelectrophoresis and inhibiting both the 5'-nucleotidase and Mg++-ATPase of this fraction. Immunofluorescent staining of tissue sections showed that the FI was derived from the parietal cell of gastric tissue and was localized to the supranuclear area of the cell. Staining of isolated rat gastric cell suspensions by FI antibodies confirmed the selectivity of the antibody and showed a polar, plasma membrane localization. FII antibodies also largely stained the parietal cells in tissue sections. In the 16 hog tissues tested, FI antibodies cross-reacted only with gastric fundus, thyroid and weakly with thymus. Immunoelectronmicroscopy showed that FI antibodies reacted strongly with the secretory membrane at the apical cell surface of the parietal cells and at the secretory canaliculi, weakly with the apical surface of the zymogen cell, and not with the basal-lateral surface of the cells. Thus, the protontranslocating ATPase is localized in the parietal cells and in the region postulated to be the site of acid secretion." @default.
- W2023200247 created "2016-06-24" @default.
- W2023200247 creator A5037799536 @default.
- W2023200247 creator A5056531508 @default.
- W2023200247 creator A5057185561 @default.
- W2023200247 creator A5066315747 @default.
- W2023200247 creator A5071583324 @default.
- W2023200247 creator A5086799917 @default.
- W2023200247 date "1979-11-01" @default.
- W2023200247 modified "2023-09-26" @default.
- W2023200247 title "Characterization of gastric mucosal membranes. X. Immunological studies of gastric (H+ + K+)-ATPase." @default.
- W2023200247 cites W1525884759 @default.
- W2023200247 cites W1566584097 @default.
- W2023200247 cites W1775749144 @default.
- W2023200247 cites W1975367964 @default.
- W2023200247 cites W1978580737 @default.
- W2023200247 cites W1998030038 @default.
- W2023200247 cites W2026391301 @default.
- W2023200247 cites W2035309872 @default.
- W2023200247 cites W2042425205 @default.
- W2023200247 cites W2058142169 @default.
- W2023200247 cites W2059433873 @default.
- W2023200247 cites W2073252544 @default.
- W2023200247 cites W2400206204 @default.
- W2023200247 cites W2421281171 @default.
- W2023200247 doi "https://doi.org/10.1083/jcb.83.2.271" @default.
- W2023200247 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2111547" @default.
- W2023200247 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/159310" @default.
- W2023200247 hasPublicationYear "1979" @default.
- W2023200247 type Work @default.
- W2023200247 sameAs 2023200247 @default.
- W2023200247 citedByCount "104" @default.
- W2023200247 countsByYear W20232002472014 @default.
- W2023200247 countsByYear W20232002472020 @default.
- W2023200247 crossrefType "journal-article" @default.
- W2023200247 hasAuthorship W2023200247A5037799536 @default.
- W2023200247 hasAuthorship W2023200247A5056531508 @default.
- W2023200247 hasAuthorship W2023200247A5057185561 @default.
- W2023200247 hasAuthorship W2023200247A5066315747 @default.
- W2023200247 hasAuthorship W2023200247A5071583324 @default.
- W2023200247 hasAuthorship W2023200247A5086799917 @default.
- W2023200247 hasBestOaLocation W20232002471 @default.
- W2023200247 hasConcept C132946820 @default.
- W2023200247 hasConcept C147483822 @default.
- W2023200247 hasConcept C153911025 @default.
- W2023200247 hasConcept C159654299 @default.
- W2023200247 hasConcept C181199279 @default.
- W2023200247 hasConcept C203014093 @default.
- W2023200247 hasConcept C23265538 @default.
- W2023200247 hasConcept C2779422922 @default.
- W2023200247 hasConcept C2779743957 @default.
- W2023200247 hasConcept C2781443798 @default.
- W2023200247 hasConcept C2911069308 @default.
- W2023200247 hasConcept C54355233 @default.
- W2023200247 hasConcept C55493867 @default.
- W2023200247 hasConcept C74864618 @default.
- W2023200247 hasConcept C86803240 @default.
- W2023200247 hasConceptScore W2023200247C132946820 @default.
- W2023200247 hasConceptScore W2023200247C147483822 @default.
- W2023200247 hasConceptScore W2023200247C153911025 @default.
- W2023200247 hasConceptScore W2023200247C159654299 @default.
- W2023200247 hasConceptScore W2023200247C181199279 @default.
- W2023200247 hasConceptScore W2023200247C203014093 @default.
- W2023200247 hasConceptScore W2023200247C23265538 @default.
- W2023200247 hasConceptScore W2023200247C2779422922 @default.
- W2023200247 hasConceptScore W2023200247C2779743957 @default.
- W2023200247 hasConceptScore W2023200247C2781443798 @default.
- W2023200247 hasConceptScore W2023200247C2911069308 @default.
- W2023200247 hasConceptScore W2023200247C54355233 @default.
- W2023200247 hasConceptScore W2023200247C55493867 @default.
- W2023200247 hasConceptScore W2023200247C74864618 @default.
- W2023200247 hasConceptScore W2023200247C86803240 @default.
- W2023200247 hasIssue "2" @default.
- W2023200247 hasLocation W20232002471 @default.
- W2023200247 hasLocation W20232002472 @default.
- W2023200247 hasLocation W20232002473 @default.
- W2023200247 hasLocation W20232002474 @default.
- W2023200247 hasOpenAccess W2023200247 @default.
- W2023200247 hasPrimaryLocation W20232002471 @default.
- W2023200247 hasRelatedWork W1518627203 @default.
- W2023200247 hasRelatedWork W1809620190 @default.
- W2023200247 hasRelatedWork W1947288861 @default.
- W2023200247 hasRelatedWork W1974856485 @default.
- W2023200247 hasRelatedWork W1979087886 @default.
- W2023200247 hasRelatedWork W2004214475 @default.
- W2023200247 hasRelatedWork W2016790735 @default.
- W2023200247 hasRelatedWork W2056374614 @default.
- W2023200247 hasRelatedWork W2060043280 @default.
- W2023200247 hasRelatedWork W2408412460 @default.
- W2023200247 hasVolume "83" @default.
- W2023200247 isParatext "false" @default.
- W2023200247 isRetracted "false" @default.
- W2023200247 magId "2023200247" @default.
- W2023200247 workType "article" @default.