FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023201005> ?p ?o ?g. }

• W2023201005 endingPage "274" @default.
• W2023201005 startingPage "266" @default.
• W2023201005 abstract "Mechanical loading of bone may be transmitted to osteocytes and osteoblasts via shear stresses at cell surfaces generated by the flow of interstitial fluid. The stimulated production of prostaglandins, which mediates some effects of mechanical loading on bone, is dependent on inducible cyclo-oxygenase 2 (COX-2) in bone cells. We examined the fluid shear stress (FSS) induction of COX-2 gene expression in immortalized MC3T3-E1 osteoblastic cells stably transfected with -371/+70 base pairs (bp) of the COX-2 5'-flanking DNA (Pluc371) and in primary osteoblasts (POBs) from calvaria of mice transgenic for Pluc371. Cells were plated on collagen-coated glass slides and subjected to steady laminar FSS in a parallel plate flow chamber. FSS, from 0.14 to 10 dynes/cm2, induced COX-2 messenger RNA (mRNA) and protein. FSS (10 dynes/cm2) induced COX-2 mRNA within 30 minutes, with peak effects at 4 h in MC3T3-E1 cells and at > or = 8 h in POBs. An inhibitor of new protein synthesis puromycin blocked the peak induction of COX-2 mRNA by FSS. COX-2 promoter activity, measured as luciferase activity, correlated with COX-2 mRNA expression in both MC3T3-E1 and POB cells. FSS induced phosphorylation of extracellular signal-regulated kinase (ERK) in MC3T3-E1 cells, with peak effects at 5 minutes. Inhibiting ERK phosphorylation with the specific inhibitor PD98059 inhibited FSS induction of COX-2 mRNA by 55-70% and FSS stimulation of luciferase activity by > or = 80% in both MC3T3-E1 and POB cells. We conclude that FSS transcriptionally induces COX-2 gene expression in osteoblasts, that the maximum induction requires new protein synthesis, and that induction occurs largely via an ERK signaling pathway." @default.
• W2023201005 created "2016-06-24" @default.
• W2023201005 creator A5000989306 @default.
• W2023201005 creator A5008051948 @default.
• W2023201005 creator A5013422566 @default.
• W2023201005 creator A5021203339 @default.
• W2023201005 creator A5024753241 @default.
• W2023201005 creator A5050735112 @default.
• W2023201005 date "2002-02-01" @default.
• W2023201005 modified "2023-10-11" @default.
• W2023201005 title "Fluid Flow Induction of Cyclo-Oxygenase 2 Gene Expression in Osteoblasts Is Dependent on an Extracellular Signal-Regulated Kinase Signaling Pathway" @default.
• W2023201005 cites W1485442945 @default.
• W2023201005 cites W1486376581 @default.
• W2023201005 cites W1603002960 @default.
• W2023201005 cites W1873184289 @default.
• W2023201005 cites W1884900523 @default.
• W2023201005 cites W1918886276 @default.
• W2023201005 cites W1932241391 @default.
• W2023201005 cites W1965240466 @default.
• W2023201005 cites W1968830328 @default.
• W2023201005 cites W1973032213 @default.
• W2023201005 cites W1975023188 @default.
• W2023201005 cites W1995819749 @default.
• W2023201005 cites W2003116598 @default.
• W2023201005 cites W2006184287 @default.
• W2023201005 cites W2007307882 @default.
• W2023201005 cites W2023987420 @default.
• W2023201005 cites W2027914516 @default.
• W2023201005 cites W2035035957 @default.
• W2023201005 cites W2039457840 @default.
• W2023201005 cites W2041970017 @default.
• W2023201005 cites W2044658581 @default.
• W2023201005 cites W2044864383 @default.
• W2023201005 cites W2052204645 @default.
• W2023201005 cites W2055658787 @default.
• W2023201005 cites W2066523609 @default.
• W2023201005 cites W2085989796 @default.
• W2023201005 cites W2087388405 @default.
• W2023201005 cites W2088602091 @default.
• W2023201005 cites W2088870579 @default.
• W2023201005 cites W2089475201 @default.
• W2023201005 cites W2090941846 @default.
• W2023201005 cites W2096925272 @default.
• W2023201005 cites W2099311149 @default.
• W2023201005 cites W2107010463 @default.
• W2023201005 cites W2122726899 @default.
• W2023201005 cites W2129324939 @default.
• W2023201005 cites W2138852525 @default.
• W2023201005 cites W2145937652 @default.
• W2023201005 cites W2152343301 @default.
• W2023201005 cites W2153057165 @default.
• W2023201005 cites W2163414758 @default.
• W2023201005 cites W2164319357 @default.
• W2023201005 cites W2164763877 @default.
• W2023201005 cites W2186137834 @default.
• W2023201005 cites W2318366640 @default.
• W2023201005 doi "https://doi.org/10.1359/jbmr.2002.17.2.266" @default.
• W2023201005 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11811557" @default.
• W2023201005 hasPublicationYear "2002" @default.
• W2023201005 type Work @default.
• W2023201005 sameAs 2023201005 @default.
• W2023201005 citedByCount "126" @default.
• W2023201005 countsByYear W20232010052012 @default.
• W2023201005 countsByYear W20232010052013 @default.
• W2023201005 countsByYear W20232010052014 @default.
• W2023201005 countsByYear W20232010052015 @default.
• W2023201005 countsByYear W20232010052016 @default.
• W2023201005 countsByYear W20232010052017 @default.
• W2023201005 countsByYear W20232010052018 @default.
• W2023201005 countsByYear W20232010052019 @default.
• W2023201005 countsByYear W20232010052020 @default.
• W2023201005 countsByYear W20232010052021 @default.
• W2023201005 countsByYear W20232010052022 @default.
• W2023201005 crossrefType "journal-article" @default.
• W2023201005 hasAuthorship W2023201005A5000989306 @default.
• W2023201005 hasAuthorship W2023201005A5008051948 @default.
• W2023201005 hasAuthorship W2023201005A5013422566 @default.
• W2023201005 hasAuthorship W2023201005A5021203339 @default.
• W2023201005 hasAuthorship W2023201005A5024753241 @default.
• W2023201005 hasAuthorship W2023201005A5050735112 @default.
• W2023201005 hasBestOaLocation W20232010051 @default.
• W2023201005 hasConcept C104317684 @default.
• W2023201005 hasConcept C105580179 @default.
• W2023201005 hasConcept C111335760 @default.
• W2023201005 hasConcept C11960822 @default.
• W2023201005 hasConcept C150194340 @default.
• W2023201005 hasConcept C153911025 @default.
• W2023201005 hasConcept C184235292 @default.
• W2023201005 hasConcept C185592680 @default.
• W2023201005 hasConcept C202751555 @default.
• W2023201005 hasConcept C2778260815 @default.
• W2023201005 hasConcept C28406088 @default.
• W2023201005 hasConcept C54009773 @default.
• W2023201005 hasConcept C55493867 @default.
• W2023201005 hasConcept C57074206 @default.
• W2023201005 hasConcept C62478195 @default.
• W2023201005 hasConcept C86803240 @default.
• W2023201005 hasConcept C95444343 @default.

• next