FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023201702> ?p ?o ?g. }

• W2023201702 endingPage "214" @default.
• W2023201702 startingPage "203" @default.
• W2023201702 abstract "As a first step towards elucidating mechanisms involved in neuroendocrine synaptogenesis, we developed a model of co-culture based on hypothalamic–intermediate pituitary interactions. Dissociated hypothalamic neurons from fetal rats at embryonic day 15 were cultured in a defined medium together with melanotrope cells of the pituitary intermediate lobe from neonatal rats. In these co-cultures, establishment of synaptic contacts between GABAergic or dopaminergic neurons and an endocrine target cell, the melanotrope cell, was studied by morphofunctional approaches. Using double immunostaining with antibodies directed against glutamate decarboxylase or tyrosine hydroxylase and α-melanocyte-stimulating hormone, we demonstrated morphological contacts between GABAergic or dopaminergic neurons and melanotrope cells as early as three days in vitro. Furthermore, using an antibody directed against synapsin I, we showed a modification of synapsin I immunoreactivity from diffuse to punctate distribution correlated with the establishment of contacts and the observation of characteristic neuroendocrine synapses by electron microscopy. These results were further confirmed by electrophysiological studies. Patch-clamp recordings demonstrated that, at six days in vitro, some melanotrope cells displayed GABAergic synaptic currents, which occurred either spontaneously and/or could be evoked chemically by 50 mM KCl or 100 μM kainate. The proportion of the melanotrope cells receiving functional synaptic inputs increased until 10 days in culture, a stage at which virtually all melanotrope cells in contact with neurons possessed functional synapses. The results presented here describe the establishment of neuroendocrine synapses in vitro, studied by combining morphofunctional and electrophysiological approaches." @default.
• W2023201702 created "2016-06-24" @default.
• W2023201702 creator A5008185976 @default.
• W2023201702 creator A5056367906 @default.
• W2023201702 creator A5059623000 @default.
• W2023201702 creator A5065356244 @default.
• W2023201702 creator A5072760094 @default.
• W2023201702 date "1996-12-01" @default.
• W2023201702 modified "2023-10-18" @default.
• W2023201702 title "Co-culture of hypothalamic neurons and melanotrope cells: a model to study synaptogenesis between central neurons and endocrine cells" @default.
• W2023201702 cites W1584569428 @default.
• W2023201702 cites W1849901991 @default.
• W2023201702 cites W1872089731 @default.
• W2023201702 cites W1966379399 @default.
• W2023201702 cites W1969219112 @default.
• W2023201702 cites W1969630044 @default.
• W2023201702 cites W1971685066 @default.
• W2023201702 cites W1974577580 @default.
• W2023201702 cites W1975372613 @default.
• W2023201702 cites W1982193506 @default.
• W2023201702 cites W1984121224 @default.
• W2023201702 cites W1989321146 @default.
• W2023201702 cites W1998225973 @default.
• W2023201702 cites W1998270315 @default.
• W2023201702 cites W2004149919 @default.
• W2023201702 cites W2004162860 @default.
• W2023201702 cites W2004963533 @default.
• W2023201702 cites W2006731169 @default.
• W2023201702 cites W2009667219 @default.
• W2023201702 cites W2009679579 @default.
• W2023201702 cites W2016394124 @default.
• W2023201702 cites W2017386826 @default.
• W2023201702 cites W2020439478 @default.
• W2023201702 cites W2029552094 @default.
• W2023201702 cites W2038713217 @default.
• W2023201702 cites W2041335497 @default.
• W2023201702 cites W2049273715 @default.
• W2023201702 cites W2049599372 @default.
• W2023201702 cites W2059319460 @default.
• W2023201702 cites W2068850166 @default.
• W2023201702 cites W2073915537 @default.
• W2023201702 cites W2074657539 @default.
• W2023201702 cites W2079265354 @default.
• W2023201702 cites W2082188651 @default.
• W2023201702 cites W2086450872 @default.
• W2023201702 cites W2089553250 @default.
• W2023201702 cites W2093656447 @default.
• W2023201702 cites W2094377611 @default.
• W2023201702 cites W2114586804 @default.
• W2023201702 cites W2120417507 @default.
• W2023201702 cites W2125483995 @default.
• W2023201702 cites W2129995902 @default.
• W2023201702 cites W2135977529 @default.
• W2023201702 cites W2137760362 @default.
• W2023201702 cites W2147613968 @default.
• W2023201702 cites W2164477294 @default.
• W2023201702 cites W4241399060 @default.
• W2023201702 doi "https://doi.org/10.1016/s0306-4522(96)00279-5" @default.
• W2023201702 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8971772" @default.
• W2023201702 hasPublicationYear "1996" @default.
• W2023201702 type Work @default.
• W2023201702 sameAs 2023201702 @default.
• W2023201702 citedByCount "10" @default.
• W2023201702 countsByYear W20232017022012 @default.
• W2023201702 countsByYear W20232017022021 @default.
• W2023201702 crossrefType "journal-article" @default.
• W2023201702 hasAuthorship W2023201702A5008185976 @default.
• W2023201702 hasAuthorship W2023201702A5056367906 @default.
• W2023201702 hasAuthorship W2023201702A5059623000 @default.
• W2023201702 hasAuthorship W2023201702A5065356244 @default.
• W2023201702 hasAuthorship W2023201702A5072760094 @default.
• W2023201702 hasConcept C126322002 @default.
• W2023201702 hasConcept C134018914 @default.
• W2023201702 hasConcept C137183658 @default.
• W2023201702 hasConcept C169760540 @default.
• W2023201702 hasConcept C17077164 @default.
• W2023201702 hasConcept C185263204 @default.
• W2023201702 hasConcept C2779177108 @default.
• W2023201702 hasConcept C29694410 @default.
• W2023201702 hasConcept C513476851 @default.
• W2023201702 hasConcept C65232495 @default.
• W2023201702 hasConcept C71924100 @default.
• W2023201702 hasConcept C86803240 @default.
• W2023201702 hasConcept C95444343 @default.
• W2023201702 hasConceptScore W2023201702C126322002 @default.
• W2023201702 hasConceptScore W2023201702C134018914 @default.
• W2023201702 hasConceptScore W2023201702C137183658 @default.
• W2023201702 hasConceptScore W2023201702C169760540 @default.
• W2023201702 hasConceptScore W2023201702C17077164 @default.
• W2023201702 hasConceptScore W2023201702C185263204 @default.
• W2023201702 hasConceptScore W2023201702C2779177108 @default.
• W2023201702 hasConceptScore W2023201702C29694410 @default.
• W2023201702 hasConceptScore W2023201702C513476851 @default.
• W2023201702 hasConceptScore W2023201702C65232495 @default.
• W2023201702 hasConceptScore W2023201702C71924100 @default.
• W2023201702 hasConceptScore W2023201702C86803240 @default.
• W2023201702 hasConceptScore W2023201702C95444343 @default.
• W2023201702 hasIssue "1" @default.

• next