Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023201910> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W2023201910 endingPage "7585" @default.
- W2023201910 startingPage "7577" @default.
- W2023201910 abstract "The EcoRV endonuclease contacts the minor groove of DNA through a peptide loop encompassing residues 67-72. This loop adapts to distorted DNA in the specific complex and to regular DNA in the nonspecific complex. Random mutagenesis had previously identified glutamine 69 as the key component of the loop and this study reports on mutants with glutamate (Q69E), lysine (Q69K), or leucine (Q69L) at this position. The mutants bound DNA specifically at the EcoRV recognition site in the presence of Ca2+, in the same manner as wild-type EcoRV. In the absence of divalent metals, Q69K and Q69L showed the same nonspecific binding as native EcoRV while Q69E failed to bind DNA. Glutamate at position 69 presumably repels nonspecific DNA whilst allowing the adaptations to specific DNA. Both Q69E and Q69K had severely impaired DNA cleavage activities, while Q69L had a steady-state k(cat) within an order of magnitude of wild-type EcoRV though its primary product was nicked DNA, in contrast to double strand breaks by wild-type EcoRV. The activity of Q69L required higher concentrations of Mg2+ than the wild-type and showed a sigmoidal dependence upon the Mg2+ concentration, indicating two metal ions per strand scission. Transient kinetics on Q69L gave lower rate constants for phosphodiester hydrolysis than wild-type EcoRV and its reaction also involved a slow conformational change preceding DNA cleavage that had no equivalent with the wild-type. Gln69 in EcoRV thus plays key roles in the adjustments of the protein to varied DNA structures and in the alignment of the catalytic functions for DNA cleavage." @default.
- W2023201910 created "2016-06-24" @default.
- W2023201910 creator A5027873958 @default.
- W2023201910 creator A5079763533 @default.
- W2023201910 date "1997-06-01" @default.
- W2023201910 modified "2023-10-17" @default.
- W2023201910 title "Kinetic Analysis of a Mutational Hot Spot in the <i>Eco</i>RV Restriction Endonuclease" @default.
- W2023201910 cites W1525252873 @default.
- W2023201910 cites W1593479774 @default.
- W2023201910 cites W163176627 @default.
- W2023201910 cites W2066921413 @default.
- W2023201910 cites W4254085062 @default.
- W2023201910 cites W75205181 @default.
- W2023201910 doi "https://doi.org/10.1021/bi970156k" @default.
- W2023201910 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9200709" @default.
- W2023201910 hasPublicationYear "1997" @default.
- W2023201910 type Work @default.
- W2023201910 sameAs 2023201910 @default.
- W2023201910 citedByCount "9" @default.
- W2023201910 crossrefType "journal-article" @default.
- W2023201910 hasAuthorship W2023201910A5027873958 @default.
- W2023201910 hasAuthorship W2023201910A5079763533 @default.
- W2023201910 hasConcept C104317684 @default.
- W2023201910 hasConcept C128319531 @default.
- W2023201910 hasConcept C143065580 @default.
- W2023201910 hasConcept C151292725 @default.
- W2023201910 hasConcept C175114707 @default.
- W2023201910 hasConcept C185592680 @default.
- W2023201910 hasConcept C207583985 @default.
- W2023201910 hasConcept C2776777567 @default.
- W2023201910 hasConcept C552990157 @default.
- W2023201910 hasConcept C55493867 @default.
- W2023201910 hasConcept C71240020 @default.
- W2023201910 hasConceptScore W2023201910C104317684 @default.
- W2023201910 hasConceptScore W2023201910C128319531 @default.
- W2023201910 hasConceptScore W2023201910C143065580 @default.
- W2023201910 hasConceptScore W2023201910C151292725 @default.
- W2023201910 hasConceptScore W2023201910C175114707 @default.
- W2023201910 hasConceptScore W2023201910C185592680 @default.
- W2023201910 hasConceptScore W2023201910C207583985 @default.
- W2023201910 hasConceptScore W2023201910C2776777567 @default.
- W2023201910 hasConceptScore W2023201910C552990157 @default.
- W2023201910 hasConceptScore W2023201910C55493867 @default.
- W2023201910 hasConceptScore W2023201910C71240020 @default.
- W2023201910 hasIssue "24" @default.
- W2023201910 hasLocation W20232019101 @default.
- W2023201910 hasLocation W20232019102 @default.
- W2023201910 hasOpenAccess W2023201910 @default.
- W2023201910 hasPrimaryLocation W20232019101 @default.
- W2023201910 hasRelatedWork W2015592000 @default.
- W2023201910 hasRelatedWork W2079391870 @default.
- W2023201910 hasRelatedWork W2111256389 @default.
- W2023201910 hasRelatedWork W2154468817 @default.
- W2023201910 hasRelatedWork W2157097395 @default.
- W2023201910 hasRelatedWork W2273114050 @default.
- W2023201910 hasRelatedWork W2273425085 @default.
- W2023201910 hasRelatedWork W4298161802 @default.
- W2023201910 hasRelatedWork W55156861 @default.
- W2023201910 hasRelatedWork W2156234534 @default.
- W2023201910 hasVolume "36" @default.
- W2023201910 isParatext "false" @default.
- W2023201910 isRetracted "false" @default.
- W2023201910 magId "2023201910" @default.
- W2023201910 workType "article" @default.