Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023209546> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2023209546 endingPage "1138" @default.
- W2023209546 startingPage "1133" @default.
- W2023209546 abstract "UDPG:thiohydroximate glucosyltransferase (EC 2.4.1.-), which catalyses the penultimate reaction in glucosinolate biosynthesis, was purified 1100-fold in 37% yield from Arabidopsis thaliana inflorescences. The enzyme possessed a native Mr of 57 800 and a pI of 4.5. At its optimum pH of 6.0, it showed a Km for UDPG of 0.27 mM. Enzyme activity was stimulated 20–45% by thiol reducing agents (e.g. 2-mercaptoethanol, l-cysteine), CaCl2, MgCl2 and MnCl2, but was greatly inhibited by ZnCl2, and CuCl2. With the exception of 1,10-phenanthroline (10 mM) which caused ca 50% inhibition, metal chelators had little effect upon glucosyltransferase activity. The sensitivity of the enzyme to several thiol blocking reagents suggests that sulphydryl groups are essential for GT activity. The purified Arabidopsis enzyme preparation lacked desulphoglucosinolate sulphotransferase activity." @default.
- W2023209546 created "2016-06-24" @default.
- W2023209546 creator A5006710285 @default.
- W2023209546 creator A5075351134 @default.
- W2023209546 date "1994-08-01" @default.
- W2023209546 modified "2023-09-27" @default.
- W2023209546 title "Partial purification and characterization of Arabidopsis thalian A UDPG:thiohydroximate glucosyltransferase" @default.
- W2023209546 cites W1599093857 @default.
- W2023209546 cites W1980174158 @default.
- W2023209546 cites W1984403617 @default.
- W2023209546 cites W1991261274 @default.
- W2023209546 cites W2020859060 @default.
- W2023209546 cites W2065896803 @default.
- W2023209546 cites W2068787094 @default.
- W2023209546 cites W2074698898 @default.
- W2023209546 cites W2100837269 @default.
- W2023209546 cites W2165639461 @default.
- W2023209546 cites W4293247451 @default.
- W2023209546 doi "https://doi.org/10.1016/s0031-9422(00)89626-0" @default.
- W2023209546 hasPublicationYear "1994" @default.
- W2023209546 type Work @default.
- W2023209546 sameAs 2023209546 @default.
- W2023209546 citedByCount "23" @default.
- W2023209546 countsByYear W20232095462016 @default.
- W2023209546 countsByYear W20232095462018 @default.
- W2023209546 crossrefType "journal-article" @default.
- W2023209546 hasAuthorship W2023209546A5006710285 @default.
- W2023209546 hasAuthorship W2023209546A5075351134 @default.
- W2023209546 hasConcept C104317684 @default.
- W2023209546 hasConcept C143065580 @default.
- W2023209546 hasConcept C181199279 @default.
- W2023209546 hasConcept C185592680 @default.
- W2023209546 hasConcept C2775988993 @default.
- W2023209546 hasConcept C2776747608 @default.
- W2023209546 hasConcept C2777858569 @default.
- W2023209546 hasConcept C2778164613 @default.
- W2023209546 hasConcept C2779201268 @default.
- W2023209546 hasConcept C2779491563 @default.
- W2023209546 hasConcept C2781324293 @default.
- W2023209546 hasConcept C28212737 @default.
- W2023209546 hasConcept C2910007386 @default.
- W2023209546 hasConcept C553450214 @default.
- W2023209546 hasConcept C55493867 @default.
- W2023209546 hasConcept C59822182 @default.
- W2023209546 hasConcept C86803240 @default.
- W2023209546 hasConceptScore W2023209546C104317684 @default.
- W2023209546 hasConceptScore W2023209546C143065580 @default.
- W2023209546 hasConceptScore W2023209546C181199279 @default.
- W2023209546 hasConceptScore W2023209546C185592680 @default.
- W2023209546 hasConceptScore W2023209546C2775988993 @default.
- W2023209546 hasConceptScore W2023209546C2776747608 @default.
- W2023209546 hasConceptScore W2023209546C2777858569 @default.
- W2023209546 hasConceptScore W2023209546C2778164613 @default.
- W2023209546 hasConceptScore W2023209546C2779201268 @default.
- W2023209546 hasConceptScore W2023209546C2779491563 @default.
- W2023209546 hasConceptScore W2023209546C2781324293 @default.
- W2023209546 hasConceptScore W2023209546C28212737 @default.
- W2023209546 hasConceptScore W2023209546C2910007386 @default.
- W2023209546 hasConceptScore W2023209546C553450214 @default.
- W2023209546 hasConceptScore W2023209546C55493867 @default.
- W2023209546 hasConceptScore W2023209546C59822182 @default.
- W2023209546 hasConceptScore W2023209546C86803240 @default.
- W2023209546 hasIssue "5" @default.
- W2023209546 hasLocation W20232095461 @default.
- W2023209546 hasOpenAccess W2023209546 @default.
- W2023209546 hasPrimaryLocation W20232095461 @default.
- W2023209546 hasRelatedWork W1989745397 @default.
- W2023209546 hasRelatedWork W2004385217 @default.
- W2023209546 hasRelatedWork W2010372900 @default.
- W2023209546 hasRelatedWork W2056002419 @default.
- W2023209546 hasRelatedWork W2071905031 @default.
- W2023209546 hasRelatedWork W2146149684 @default.
- W2023209546 hasRelatedWork W2161295757 @default.
- W2023209546 hasRelatedWork W2287018658 @default.
- W2023209546 hasRelatedWork W2984947154 @default.
- W2023209546 hasRelatedWork W950805422 @default.
- W2023209546 hasVolume "36" @default.
- W2023209546 isParatext "false" @default.
- W2023209546 isRetracted "false" @default.
- W2023209546 magId "2023209546" @default.
- W2023209546 workType "article" @default.