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- W2023211456 abstract "Many types of cancer and neurodegenerative disease are caused by abnormalities and variations in the genome. Genetic variations may vary from a single DNA nucleotide mutation to total loss of chromosomes. We have designed a high resolution imaging technique for determining structural variations of genes related to genetic diseases. Our technique relies on successfully stretching and linearizing single DNA molecules close to their contour length and mapping them using high resolution imaging techniques. Single-Molecule High REsolution Colocalization (SHREC) and Single-molecule High-Resolution Imaging with photobleaching (SHRImP) methods have been used to measure distances between two fluorophores that are closer than the Rayleigh limit control [Gordon, PNAS,2004; Churchman, PNAS, 2005]. Combining the two techniques and applying them to DNA, we have successfully measured the distance between fluorophores separated by 10-100 nm. We have also successfully shown 7 restrictions sites of Nb.BbvC I endonuclease enzyme on lambda DNA. We also report an improvement in optical DNA mapping by labeling two unique sequence motifs (GCTGGG, GGAACTT) with two different single color fluorophores. We generate a detailed map with 100 bp resolution on BAC DNA which allows us to identify genomic variations less than sub-kilobase level." @default.
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- W2023211456 date "2012-01-01" @default.
- W2023211456 modified "2023-09-30" @default.
- W2023211456 title "Advance High Resolution DNA Mapping Technique to Identify Genomic Variations" @default.
- W2023211456 doi "https://doi.org/10.1016/j.bpj.2011.11.2295" @default.
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