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- W2023222253 abstract "An efficient preparation of Periplaneta americana nymphae allergen, Cr PI (54 kDa) is described. It was expressed as a GST-tag fusion protein in Escherichia coli, strain BL21 (DE3). Expression of recombinant Cr PI (rCr PI), denaturation/ renaturation of the inclusion bodies and the effects of protein and L-arginine concentration on inclusion body aggregation were optimized. The fusion protein was purified by affinity chromatography and size exclusion chromatography, and Cr PI fusion protein was purified to > 95%. rCr PI bound strongly to IgE in the sera of individuals with cockroach allergies as shown by western blot and ELISA. Highly refolded and purified recombinant protein was obtained, providing a basis for the large-scale preparation of Cr PI allergen. Keywords: Periplaneta americana, Cr PI allergen, high-level expression, inclusion bodies, immunological characterization" @default.
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- W2023222253 date "2007-09-01" @default.
- W2023222253 modified "2023-09-27" @default.
- W2023222253 title "Expression, Purification, and Immunological Characterization of Cr PI" @default.
- W2023222253 doi "https://doi.org/10.2174/092986607782110266" @default.
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