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• W2023231829 endingPage "692" @default.
• W2023231829 startingPage "679" @default.
• W2023231829 abstract "To study the elements involved in the pituitary specific transcriptional regulation of the tilapia prolactin I gene (tiPRL I), we have cloned and entirely sequenced a 3.4-kb genomic fragment immediately upstream from the first exon. In footprinting experiments, three tilapia sequences are protected from DNase I digestion by rat pituitary extracts (base pair coordinates −643 to −593, −160 to −111, and −73 to −46). Computer analysis of the nucleotide sequence reveals significant homology to mammalian binding sites for Pit-1, a transcription factor that is known to mediate pituitary-specific expression of the PRL genes in mammals. The tiPRL I 5′-flanking sequences can direct transient expression of a linked luciferase reporter gene in transfected rat pituitary cell lines and tilapia pituitary primary cell cultures. Transient expression experiments with 5′-deletion mutants reveal three regulatory regions. Two have a stimulatory effect on transcription and one an inhibitory effect. Electrophoretic mobility-shift assays (EMSA) demonstrate that the rat Pit-1 factor specifically binds to tilapia DNA sequences. Several such tilapia Pit-1 binding sites mediate activation of a linked heterologous promoter in transfected rat and tilapia pituitary cells. As evidenced by EMSA, a Pit-1-like protein is present in tilapia pituitary extracts. All these data point to a high conservation of the molecular mechanisms involved in pituitary-specific expression of the PRL genes in vertebrates." @default.
• W2023231829 created "2016-06-24" @default.
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• W2023231829 date "1996-08-01" @default.
• W2023231829 modified "2023-09-27" @default.
• W2023231829 title "The Tilapia Prolactin I Gene: Evolutionary Conservation of the Regulatory Elements Directing Pituitary-Specific Expression" @default.
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• W2023231829 doi "https://doi.org/10.1089/dna.1996.15.679" @default.
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