Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023238422> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2023238422 endingPage "102" @default.
- W2023238422 startingPage "93" @default.
- W2023238422 abstract "We review a method termed PCR Amplification of Specific Alleles (PASA), a generally applicable technique for the detection of known point mutations, small deletions and insertions, polymorphisms and other sequence variations. PASA is a modification of PCR that depends on the synthesis of a PCR oligonucleotide primer that precisely matches with one of the alleles but mismatches with the other. When the mismatch occurs near the 3' end of the PCR primer, amplification is inefficient. Therefore, preferential amplification of the perfectly matched allele is obtained. The method should be generally applicable as our results indicate that with proper optimization all possible alleles can be reliably distinguished. The ease and technical simplicity of PASA make genetic analyses more accessible. PASA can be also adapted to accommodate specific requirements and can be extended by incorporating other techniques. Moreover, PASA shows promise for population screening because the technique is rapid, highly reproducible, inexpensive, nonisotopic, and amendable to automation." @default.
- W2023238422 created "2016-06-24" @default.
- W2023238422 creator A5063106224 @default.
- W2023238422 creator A5086533536 @default.
- W2023238422 date "1993-07-01" @default.
- W2023238422 modified "2023-09-28" @default.
- W2023238422 title "PCR amplification of specific alleles: Rapid detection of known mutations and polymorphisms" @default.
- W2023238422 cites W1760369460 @default.
- W2023238422 cites W1968435166 @default.
- W2023238422 cites W1979388953 @default.
- W2023238422 cites W1987389293 @default.
- W2023238422 cites W2011353459 @default.
- W2023238422 cites W2043900769 @default.
- W2023238422 cites W2064063117 @default.
- W2023238422 cites W2078178142 @default.
- W2023238422 cites W2082804336 @default.
- W2023238422 cites W2147184989 @default.
- W2023238422 doi "https://doi.org/10.1016/0027-5107(93)90211-w" @default.
- W2023238422 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7686270" @default.
- W2023238422 hasPublicationYear "1993" @default.
- W2023238422 type Work @default.
- W2023238422 sameAs 2023238422 @default.
- W2023238422 citedByCount "115" @default.
- W2023238422 countsByYear W20232384222012 @default.
- W2023238422 countsByYear W20232384222013 @default.
- W2023238422 countsByYear W20232384222014 @default.
- W2023238422 countsByYear W20232384222015 @default.
- W2023238422 countsByYear W20232384222016 @default.
- W2023238422 countsByYear W20232384222017 @default.
- W2023238422 countsByYear W20232384222018 @default.
- W2023238422 countsByYear W20232384222019 @default.
- W2023238422 countsByYear W20232384222020 @default.
- W2023238422 countsByYear W20232384222021 @default.
- W2023238422 countsByYear W20232384222022 @default.
- W2023238422 countsByYear W20232384222023 @default.
- W2023238422 crossrefType "journal-article" @default.
- W2023238422 hasAuthorship W2023238422A5063106224 @default.
- W2023238422 hasAuthorship W2023238422A5086533536 @default.
- W2023238422 hasConcept C104317684 @default.
- W2023238422 hasConcept C129312508 @default.
- W2023238422 hasConcept C135763542 @default.
- W2023238422 hasConcept C176944494 @default.
- W2023238422 hasConcept C178790620 @default.
- W2023238422 hasConcept C180754005 @default.
- W2023238422 hasConcept C185592680 @default.
- W2023238422 hasConcept C2777563447 @default.
- W2023238422 hasConcept C31467283 @default.
- W2023238422 hasConcept C33935397 @default.
- W2023238422 hasConcept C49105822 @default.
- W2023238422 hasConcept C501734568 @default.
- W2023238422 hasConcept C54355233 @default.
- W2023238422 hasConcept C70721500 @default.
- W2023238422 hasConcept C86803240 @default.
- W2023238422 hasConceptScore W2023238422C104317684 @default.
- W2023238422 hasConceptScore W2023238422C129312508 @default.
- W2023238422 hasConceptScore W2023238422C135763542 @default.
- W2023238422 hasConceptScore W2023238422C176944494 @default.
- W2023238422 hasConceptScore W2023238422C178790620 @default.
- W2023238422 hasConceptScore W2023238422C180754005 @default.
- W2023238422 hasConceptScore W2023238422C185592680 @default.
- W2023238422 hasConceptScore W2023238422C2777563447 @default.
- W2023238422 hasConceptScore W2023238422C31467283 @default.
- W2023238422 hasConceptScore W2023238422C33935397 @default.
- W2023238422 hasConceptScore W2023238422C49105822 @default.
- W2023238422 hasConceptScore W2023238422C501734568 @default.
- W2023238422 hasConceptScore W2023238422C54355233 @default.
- W2023238422 hasConceptScore W2023238422C70721500 @default.
- W2023238422 hasConceptScore W2023238422C86803240 @default.
- W2023238422 hasIssue "1" @default.
- W2023238422 hasLocation W20232384221 @default.
- W2023238422 hasLocation W20232384222 @default.
- W2023238422 hasOpenAccess W2023238422 @default.
- W2023238422 hasPrimaryLocation W20232384221 @default.
- W2023238422 hasRelatedWork W121700474 @default.
- W2023238422 hasRelatedWork W17396297 @default.
- W2023238422 hasRelatedWork W1986135054 @default.
- W2023238422 hasRelatedWork W2010208430 @default.
- W2023238422 hasRelatedWork W2039392581 @default.
- W2023238422 hasRelatedWork W2054973080 @default.
- W2023238422 hasRelatedWork W2057739827 @default.
- W2023238422 hasRelatedWork W2080644877 @default.
- W2023238422 hasRelatedWork W2115129295 @default.
- W2023238422 hasRelatedWork W2376152126 @default.
- W2023238422 hasVolume "288" @default.
- W2023238422 isParatext "false" @default.
- W2023238422 isRetracted "false" @default.
- W2023238422 magId "2023238422" @default.
- W2023238422 workType "article" @default.