FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023243346> ?p ?o ?g. }

• W2023243346 endingPage "214" @default.
• W2023243346 startingPage "208" @default.
• W2023243346 abstract "Instability of the Microsatellite DNA Instability (MSI) and Loss of Heterozygosity (LOH) have been previously detected in sputum cells of COPD patients. However, the particular cell subpopulation exhibiting genetic instability in COPD was uncertain. The aim of this study was to determine which cell type expresses Microsatellite DNA Instability in sputum and BALF samples from COPD patients.Thirty-five COPD patients and 30 non-COPD smokers were studied. Sputum was induced from 20 COPD patients and 20 non-COPD smokers and BALF was obtained from 15 COPD patients and 10 non-COPD smokers. The sputum cell pellet and BALF samples were processed using immunomagnetic technology to separate antibody-specific cell subpopulations, using CD45+ for leukocytes, Epithelial enrich (MACS) for sputum epithelial cells and HEA-human epithelial antigen-(Dynal) for BAL epithelial cells. Microsatellite DNA amplification was performed using specific primers, namely G29802, D6S2223, D6S344, D6S263, D5S207, D13S71, RH70958, and D17S250. The presence of MSI and/or LOH was analyzed with LI-COR Saga GT Microsatellite Analysis Software.None of the non-COPD smokers exhibited any genetic alteration. MSI and LOH were found in 15 cases (8 MSI and 7 LOH) in sputum and BAL samples. MSI and/or LOH were revealed only in the epithelial barrier cells. LOH was detected in D5S207, D6S344, G29802 and D17S250 microsatellite markers, while MSI in D13S71, D5S207 and D6S344. The entire leukocyte subpopulation exhibited no genetic alteration.Our results support the hypothesis that chronic inflammation and oxidative burden in COPD can lead to DNA damage of the lung epithelial barrier cells, detected at the Microsatellite DNA level. Further studies are required to investigate the significance of these findings in the pathogenesis of COPD." @default.
• W2023243346 created "2016-06-24" @default.
• W2023243346 creator A5000811370 @default.
• W2023243346 creator A5002438986 @default.
• W2023243346 creator A5019499699 @default.
• W2023243346 creator A5041786712 @default.
• W2023243346 creator A5042459782 @default.
• W2023243346 creator A5044010028 @default.
• W2023243346 creator A5088346886 @default.
• W2023243346 date "2010-06-01" @default.
• W2023243346 modified "2023-09-26" @default.
• W2023243346 title "Somatic DNA alterations in lung epithelial barrier cells in COPD patients" @default.
• W2023243346 cites W1970459532 @default.
• W2023243346 cites W1981617284 @default.
• W2023243346 cites W1999549209 @default.
• W2023243346 cites W2000526989 @default.
• W2023243346 cites W2022912717 @default.
• W2023243346 cites W2027130980 @default.
• W2023243346 cites W2028979096 @default.
• W2023243346 cites W2034714392 @default.
• W2023243346 cites W2045616259 @default.
• W2023243346 cites W2048179657 @default.
• W2023243346 cites W2059808605 @default.
• W2023243346 cites W2060436620 @default.
• W2023243346 cites W2077799959 @default.
• W2023243346 cites W2085812956 @default.
• W2023243346 cites W2087923650 @default.
• W2023243346 cites W2092156182 @default.
• W2023243346 cites W2096624811 @default.
• W2023243346 cites W2096739766 @default.
• W2023243346 cites W2097664337 @default.
• W2023243346 cites W2108876251 @default.
• W2023243346 cites W2111156631 @default.
• W2023243346 cites W2119363473 @default.
• W2023243346 cites W2127850323 @default.
• W2023243346 cites W2132383396 @default.
• W2023243346 cites W2146599180 @default.
• W2023243346 cites W2149172272 @default.
• W2023243346 cites W2151559543 @default.
• W2023243346 cites W2154023242 @default.
• W2023243346 cites W2166432084 @default.
• W2023243346 cites W42434366 @default.
• W2023243346 doi "https://doi.org/10.1016/j.pupt.2009.12.001" @default.
• W2023243346 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20040382" @default.
• W2023243346 hasPublicationYear "2010" @default.
• W2023243346 type Work @default.
• W2023243346 sameAs 2023243346 @default.
• W2023243346 citedByCount "16" @default.
• W2023243346 countsByYear W20232433462012 @default.
• W2023243346 countsByYear W20232433462013 @default.
• W2023243346 countsByYear W20232433462014 @default.
• W2023243346 countsByYear W20232433462015 @default.
• W2023243346 countsByYear W20232433462016 @default.
• W2023243346 countsByYear W20232433462017 @default.
• W2023243346 countsByYear W20232433462020 @default.
• W2023243346 countsByYear W20232433462022 @default.
• W2023243346 crossrefType "journal-article" @default.
• W2023243346 hasAuthorship W2023243346A5000811370 @default.
• W2023243346 hasAuthorship W2023243346A5002438986 @default.
• W2023243346 hasAuthorship W2023243346A5019499699 @default.
• W2023243346 hasAuthorship W2023243346A5041786712 @default.
• W2023243346 hasAuthorship W2023243346A5042459782 @default.
• W2023243346 hasAuthorship W2023243346A5044010028 @default.
• W2023243346 hasAuthorship W2023243346A5088346886 @default.
• W2023243346 hasConcept C104317684 @default.
• W2023243346 hasConcept C126322002 @default.
• W2023243346 hasConcept C142724271 @default.
• W2023243346 hasConcept C143589142 @default.
• W2023243346 hasConcept C180754005 @default.
• W2023243346 hasConcept C203014093 @default.
• W2023243346 hasConcept C2776301714 @default.
• W2023243346 hasConcept C2776780178 @default.
• W2023243346 hasConcept C2777714996 @default.
• W2023243346 hasConcept C2779767149 @default.
• W2023243346 hasConcept C2781069245 @default.
• W2023243346 hasConcept C54355233 @default.
• W2023243346 hasConcept C61320498 @default.
• W2023243346 hasConcept C71924100 @default.
• W2023243346 hasConcept C86803240 @default.
• W2023243346 hasConceptScore W2023243346C104317684 @default.
• W2023243346 hasConceptScore W2023243346C126322002 @default.
• W2023243346 hasConceptScore W2023243346C142724271 @default.
• W2023243346 hasConceptScore W2023243346C143589142 @default.
• W2023243346 hasConceptScore W2023243346C180754005 @default.
• W2023243346 hasConceptScore W2023243346C203014093 @default.
• W2023243346 hasConceptScore W2023243346C2776301714 @default.
• W2023243346 hasConceptScore W2023243346C2776780178 @default.
• W2023243346 hasConceptScore W2023243346C2777714996 @default.
• W2023243346 hasConceptScore W2023243346C2779767149 @default.
• W2023243346 hasConceptScore W2023243346C2781069245 @default.
• W2023243346 hasConceptScore W2023243346C54355233 @default.
• W2023243346 hasConceptScore W2023243346C61320498 @default.
• W2023243346 hasConceptScore W2023243346C71924100 @default.
• W2023243346 hasConceptScore W2023243346C86803240 @default.
• W2023243346 hasIssue "3" @default.
• W2023243346 hasLocation W20232433461 @default.
• W2023243346 hasLocation W20232433462 @default.
• W2023243346 hasOpenAccess W2023243346 @default.

• next