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• W2023243934 abstract "The production and characterization of a cell line for quantitative HIV-1 Tat function and T cell activation assays is described. 1G5 is a clonal cell line derived from Jurkat T cells stably transfected with a luciferase gene driven by an HIV-1 long terminal repeat (HIV-LTR). The 1G5 clone was selected for low basal luciferase activity, susceptibility to HIV infection, and high responsiveness to Tat and T cell activation signals. A 10 to 1000-fold increase in luciferase activity after transfection or infection with tat-expressing vectors or HIV was observed. Equivalent levels of expression were detected after stimulation with T cell mitogens. The characteristics of 1G5 make it a valuable reagent for studies of HIV infection, HIV regulatory agents, and other T cell or HIV -activating factors, and for screening potential anti-HIV therapeutic agents." @default.
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• W2023243934 date "1994-03-01" @default.
• W2023243934 modified "2023-10-18" @default.
• W2023243934 title "A Sensitive Reporter Cell Line for HIV-1<i>tat</i>Activity, HIV-1 Inhibitors, and T Cell Activation Effects" @default.
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• W2023243934 doi "https://doi.org/10.1089/aid.1994.10.295" @default.
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