Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023245320> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2023245320 endingPage "107" @default.
- W2023245320 startingPage "105" @default.
- W2023245320 abstract "► Report of methods to clarify significance of amino acid changes in HSV-1 TK gene. ► Recombinant proteins were synthesized after cloning and site-directed mutagenesis. ► A novel test for measuring phophorylation activity of HSV-1 TK was evaluated. ► Significance of seven amino acid substitutions for acyclovir resistance was clarified. The analysis of the viral thymidine kinase (TK) genotype is of rising significance for testing resistance of herpes simplex virus (HSV) to antivirals especially acyclovir. However, numerous of the described amino acid (aa) substitutions are diagnostically less conclusive because of the pronounced natural polymorphism of this gene. In this study, several aa substitutions in the TK sequence of HSV-1 with unclear significance for resistance were analyzed by expression of recombinant TK proteins and determination of enzymatic activity on the basis of an enzyme linked immunosorbent assay using bromodeoxyuridine (BrdU) as TK substrate. The recombinant TK wild-type protein resulted in high TK activity and TK mutant with stop of translation showed negative results. The recombinant TK proteins containing the aa substitutions R41H or V348I had high phosphorylation activities suggesting most likely natural gene polymorphisms. By contrast, the aa changes Y53H, L139V, R163H, L298A and L315S were accompanied by negative or weakly positive TK activities indicating resistance association. In conclusion, the combination of methods described here represents a useful tool to evaluate the significance of aa substitutions for resistance of clinical HSV-1 strains." @default.
- W2023245320 created "2016-06-24" @default.
- W2023245320 creator A5011977576 @default.
- W2023245320 creator A5015605375 @default.
- W2023245320 creator A5072663690 @default.
- W2023245320 creator A5074207767 @default.
- W2023245320 creator A5079008889 @default.
- W2023245320 creator A5080257805 @default.
- W2023245320 creator A5082841663 @default.
- W2023245320 date "2012-11-01" @default.
- W2023245320 modified "2023-09-27" @default.
- W2023245320 title "Significance of amino acid substitutions in the thymidine kinase gene of herpes simplex virus type 1 for resistance" @default.
- W2023245320 cites W1976409125 @default.
- W2023245320 cites W1983142184 @default.
- W2023245320 cites W1998909555 @default.
- W2023245320 cites W2009455155 @default.
- W2023245320 cites W2018863083 @default.
- W2023245320 cites W2046801142 @default.
- W2023245320 cites W2084375481 @default.
- W2023245320 cites W2089824168 @default.
- W2023245320 cites W2090765690 @default.
- W2023245320 cites W2108795035 @default.
- W2023245320 cites W2112847184 @default.
- W2023245320 cites W2113417823 @default.
- W2023245320 cites W2152809429 @default.
- W2023245320 cites W2155275691 @default.
- W2023245320 doi "https://doi.org/10.1016/j.antiviral.2012.08.001" @default.
- W2023245320 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22906425" @default.
- W2023245320 hasPublicationYear "2012" @default.
- W2023245320 type Work @default.
- W2023245320 sameAs 2023245320 @default.
- W2023245320 citedByCount "23" @default.
- W2023245320 countsByYear W20232453202013 @default.
- W2023245320 countsByYear W20232453202014 @default.
- W2023245320 countsByYear W20232453202015 @default.
- W2023245320 countsByYear W20232453202016 @default.
- W2023245320 countsByYear W20232453202017 @default.
- W2023245320 countsByYear W20232453202018 @default.
- W2023245320 countsByYear W20232453202019 @default.
- W2023245320 countsByYear W20232453202021 @default.
- W2023245320 crossrefType "journal-article" @default.
- W2023245320 hasAuthorship W2023245320A5011977576 @default.
- W2023245320 hasAuthorship W2023245320A5015605375 @default.
- W2023245320 hasAuthorship W2023245320A5072663690 @default.
- W2023245320 hasAuthorship W2023245320A5074207767 @default.
- W2023245320 hasAuthorship W2023245320A5079008889 @default.
- W2023245320 hasAuthorship W2023245320A5080257805 @default.
- W2023245320 hasAuthorship W2023245320A5082841663 @default.
- W2023245320 hasConcept C104317684 @default.
- W2023245320 hasConcept C143065580 @default.
- W2023245320 hasConcept C153911025 @default.
- W2023245320 hasConcept C159047783 @default.
- W2023245320 hasConcept C16318435 @default.
- W2023245320 hasConcept C2522874641 @default.
- W2023245320 hasConcept C2776147627 @default.
- W2023245320 hasConcept C2781196997 @default.
- W2023245320 hasConcept C40767141 @default.
- W2023245320 hasConcept C515207424 @default.
- W2023245320 hasConcept C55493867 @default.
- W2023245320 hasConcept C86803240 @default.
- W2023245320 hasConceptScore W2023245320C104317684 @default.
- W2023245320 hasConceptScore W2023245320C143065580 @default.
- W2023245320 hasConceptScore W2023245320C153911025 @default.
- W2023245320 hasConceptScore W2023245320C159047783 @default.
- W2023245320 hasConceptScore W2023245320C16318435 @default.
- W2023245320 hasConceptScore W2023245320C2522874641 @default.
- W2023245320 hasConceptScore W2023245320C2776147627 @default.
- W2023245320 hasConceptScore W2023245320C2781196997 @default.
- W2023245320 hasConceptScore W2023245320C40767141 @default.
- W2023245320 hasConceptScore W2023245320C515207424 @default.
- W2023245320 hasConceptScore W2023245320C55493867 @default.
- W2023245320 hasConceptScore W2023245320C86803240 @default.
- W2023245320 hasIssue "2" @default.
- W2023245320 hasLocation W20232453201 @default.
- W2023245320 hasLocation W20232453202 @default.
- W2023245320 hasOpenAccess W2023245320 @default.
- W2023245320 hasPrimaryLocation W20232453201 @default.
- W2023245320 hasRelatedWork W1972241043 @default.
- W2023245320 hasRelatedWork W1993339219 @default.
- W2023245320 hasRelatedWork W2017400346 @default.
- W2023245320 hasRelatedWork W2023629155 @default.
- W2023245320 hasRelatedWork W2043158748 @default.
- W2023245320 hasRelatedWork W2049265285 @default.
- W2023245320 hasRelatedWork W2102565620 @default.
- W2023245320 hasRelatedWork W2126232866 @default.
- W2023245320 hasRelatedWork W2162468440 @default.
- W2023245320 hasRelatedWork W2922493318 @default.
- W2023245320 hasVolume "96" @default.
- W2023245320 isParatext "false" @default.
- W2023245320 isRetracted "false" @default.
- W2023245320 magId "2023245320" @default.
- W2023245320 workType "article" @default.