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- W2023248536 abstract "A technic was developed which resulted in preparations containing many mitotic divisions with chromosomes well fixed and stained, rod-shaped, and spread throughout the cell. This technic has given good results with guayule (Parthenium argentatum), Crepis, Allium, Pisum, Lycopersicon, Tradescantia, and other plants. Material is prefixed in a saturated solution of paradichlorobenzene for 1-4 hours, fixed in 65% acetic acid (or other suitable fixative) for 12-24 hours, hydrolyzed in 10% HCl for 10-30 minutes at 60° C, rinsed in water, transferred to a drop of 45% acetic acid on a slide, and smeared and stained in aceto-orcein. The preparation may be made permanent by separating slide and cover glass in 1 part glacial acetic acid to 1 part absolute alcohol, putting them in absolute alcohol, and then recombining them with a drop of euparol." @default.
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- W2023248536 date "1945-01-01" @default.
- W2023248536 modified "2023-09-26" @default.
- W2023248536 title "Prefixing with Paradichlorobenzene to Facilitate Chromosome Study" @default.
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- W2023248536 doi "https://doi.org/10.3109/10520294509107146" @default.
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