Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023249566> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2023249566 abstract "The peripheral root cap cells of corn (cv. SX-17A) secrete a fucose-rich, high molecular weight, polysaccharide slime via the dictyosome pathway. To study the synthesis of this polysaccharide, a technique for isolating and assaying GDP-fucose:polysaccharide fucosyl transferase activity was developed. Corn roots were excised from germinated seeds, incubated 12 hours at 10 C in water, and ground in 100 millimolar Tris or Pipes buffer (pH 7.0) with or without 0.5 molar sucrose. The membrane-bound enzyme was solubilized by sonication in the presence of 2 molar urea and 1.5% (v/v) Triton X-100 and assayed by monitoring the incorporation of GDP-[(14)C]fucose into endogenous acceptors. Optimum enzyme activity is expressed at pH 7.0 and 30 C in the presence of 0.8% (v/v) Triton X-100. The enzyme does not require divalent cations for activation and is inhibited by concentrations of MnCl(2) or MgCl(2) greater than 1 millimolar. Corn root cap slime will serve as an exogenous acceptor for the enzyme if it is first hydrolyzed in 5 millimolar trifluoroacetic acid for 60 minutes at 18 pounds per square inch, 121 C. This procedure prepares the acceptor by removing terminal fucose residues from the slime molecule. Kinetics of fucose release during hydrolysis of native slime and in vitro synthesized product suggests that the two polymers possess similar linkages to fucose." @default.
- W2023249566 created "2016-06-24" @default.
- W2023249566 creator A5005347875 @default.
- W2023249566 creator A5008194971 @default.
- W2023249566 date "1979-12-01" @default.
- W2023249566 modified "2023-09-27" @default.
- W2023249566 title "Characterization of GDP-Fucose" @default.
- W2023249566 doi "https://doi.org/10.1104/pp.64.6.909" @default.
- W2023249566 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/543163" @default.
- W2023249566 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16661104" @default.
- W2023249566 hasPublicationYear "1979" @default.
- W2023249566 type Work @default.
- W2023249566 sameAs 2023249566 @default.
- W2023249566 citedByCount "7" @default.
- W2023249566 countsByYear W20232495662016 @default.
- W2023249566 crossrefType "journal-article" @default.
- W2023249566 hasAuthorship W2023249566A5005347875 @default.
- W2023249566 hasAuthorship W2023249566A5008194971 @default.
- W2023249566 hasBestOaLocation W20232495662 @default.
- W2023249566 hasConcept C100817775 @default.
- W2023249566 hasConcept C106827334 @default.
- W2023249566 hasConcept C108625454 @default.
- W2023249566 hasConcept C150487720 @default.
- W2023249566 hasConcept C178790620 @default.
- W2023249566 hasConcept C181199279 @default.
- W2023249566 hasConcept C185592680 @default.
- W2023249566 hasConcept C18903297 @default.
- W2023249566 hasConcept C2776841590 @default.
- W2023249566 hasConcept C2777289219 @default.
- W2023249566 hasConcept C43617362 @default.
- W2023249566 hasConcept C55493867 @default.
- W2023249566 hasConcept C74884574 @default.
- W2023249566 hasConcept C86803240 @default.
- W2023249566 hasConcept C94412978 @default.
- W2023249566 hasConceptScore W2023249566C100817775 @default.
- W2023249566 hasConceptScore W2023249566C106827334 @default.
- W2023249566 hasConceptScore W2023249566C108625454 @default.
- W2023249566 hasConceptScore W2023249566C150487720 @default.
- W2023249566 hasConceptScore W2023249566C178790620 @default.
- W2023249566 hasConceptScore W2023249566C181199279 @default.
- W2023249566 hasConceptScore W2023249566C185592680 @default.
- W2023249566 hasConceptScore W2023249566C18903297 @default.
- W2023249566 hasConceptScore W2023249566C2776841590 @default.
- W2023249566 hasConceptScore W2023249566C2777289219 @default.
- W2023249566 hasConceptScore W2023249566C43617362 @default.
- W2023249566 hasConceptScore W2023249566C55493867 @default.
- W2023249566 hasConceptScore W2023249566C74884574 @default.
- W2023249566 hasConceptScore W2023249566C86803240 @default.
- W2023249566 hasConceptScore W2023249566C94412978 @default.
- W2023249566 hasLocation W20232495661 @default.
- W2023249566 hasLocation W20232495662 @default.
- W2023249566 hasLocation W20232495663 @default.
- W2023249566 hasOpenAccess W2023249566 @default.
- W2023249566 hasPrimaryLocation W20232495661 @default.
- W2023249566 hasRelatedWork W1484107064 @default.
- W2023249566 hasRelatedWork W1539113222 @default.
- W2023249566 hasRelatedWork W1542218170 @default.
- W2023249566 hasRelatedWork W1584153502 @default.
- W2023249566 hasRelatedWork W1966046944 @default.
- W2023249566 hasRelatedWork W1971000232 @default.
- W2023249566 hasRelatedWork W1971861339 @default.
- W2023249566 hasRelatedWork W1979941909 @default.
- W2023249566 hasRelatedWork W1991459616 @default.
- W2023249566 hasRelatedWork W1992693782 @default.
- W2023249566 hasRelatedWork W2011365361 @default.
- W2023249566 hasRelatedWork W2012191503 @default.
- W2023249566 hasRelatedWork W2020859060 @default.
- W2023249566 hasRelatedWork W2021365592 @default.
- W2023249566 hasRelatedWork W2022470604 @default.
- W2023249566 hasRelatedWork W2043072818 @default.
- W2023249566 hasRelatedWork W2069137313 @default.
- W2023249566 hasRelatedWork W2088384668 @default.
- W2023249566 hasRelatedWork W2094664753 @default.
- W2023249566 hasRelatedWork W2098255923 @default.
- W2023249566 isParatext "false" @default.
- W2023249566 isRetracted "false" @default.
- W2023249566 magId "2023249566" @default.
- W2023249566 workType "article" @default.