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- W2023250076 abstract "In the present study, a novel assay for screening inhibitors of Cu(II) ion/adrenaline-mediated oxidative modification of N-terminal amyloid β (Aβ) peptides was developed using liquid chromatography with mass spectrometry (LC/MS). The physiological condition of Cu(II) ion/adrenaline in buffer (pH 7.4) at 37 °C for 90 min revealed a specific modification of N-terminal Aβ peptides, such as Aβ1−6, Aβ1−40, and Aβ1−42, using trypsin digestion and LC/MS detection of the modified Aβ peptide. When this oxidative modification of the shorter N-terminal Aβ1−6 was subjected to LC/MS, single charged ions from native peptide ([M + H]+, m/z 774) were observed at m/z 729 and 685, corresponding to a decrease in mass of 45 and 89 Da, respectively, as compared with the original peptide. To determine the effect of specific antioxidants, a screening assay to find inhibitors of Cu(II) ion/adrenaline-mediated oxidation was developed based on the response ratio of m/z 685 to 774. LC/MS detection of the modified peptides allowed us to identify antioxidants that inhibit oxidative modification of Aβ1−6 model peptide. The oxidative modification of Aβ1−6 was inhibited by curcumin but not an isoflavone or catechin mixture or saponin or capsaicin, revealing a clear difference between antioxidants that inhibit oxidative modification and other antioxidants. This novel assay may allow for the identification of antioxidants that protect against oxidative modification of Aβ and other proteins related to oxidative stress by adrenaline and Cu(II) ions under normal physiologic conditions." @default.
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- W2023250076 date "2010-08-13" @default.
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- W2023250076 title "Simple and Novel Screening Assay of Natural Antioxidants for Cu(II) Ion/Adrenaline-Mediated Oxidation of N-Terminal Amyloid β by Liquid Chromatography/Mass Spectrometry" @default.
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- W2023250076 doi "https://doi.org/10.1021/jf101661w" @default.
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