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• W2023250436 endingPage "e1002435" @default.
• W2023250436 startingPage "e1002435" @default.
• W2023250436 abstract "Proteases and protease inhibitors have been identified in the ejaculates of animal taxa ranging from invertebrates to mammals and form a major protein class among Drosophila melanogaster seminal fluid proteins (SFPs). Other than a single protease cascade in mammals that regulates seminal clot liquefaction, no proteolytic cascades (i.e. pathways with at least two proteases acting in sequence) have been identified in seminal fluids. In Drosophila, SFPs are transferred to females during mating and, together with sperm, are necessary for the many post-mating responses elicited in females. Though several SFPs are proteolytically cleaved either during or after mating, virtually nothing is known about the proteases involved in these cleavage events or the physiological consequences of proteolytic activity in the seminal fluid on the female. Here, we present evidence that a protease cascade acts in the seminal fluid of Drosophila during and after mating. Using RNAi to knock down expression of the SFP CG10586, a predicted serine protease, we show that it acts upstream of the SFP CG11864, a predicted astacin protease, to process SFPs involved in ovulation and sperm entry into storage. We also show that knockdown of CG10586 leads to lower levels of egg laying, higher rates of sexual receptivity to subsequent males, and abnormal sperm usage patterns, processes that are independent of CG11864. The long-term phenotypes of females mated to CG10586 knockdown males are similar to those of females that fail to store sex peptide, an important elicitor of long-term post-mating responses, and indicate a role for CG10586 in regulating sex peptide. These results point to an important role for proteolysis among insect SFPs and suggest that protease cascades may be a mechanism for precise temporal regulation of multiple post-mating responses in females." @default.
• W2023250436 created "2016-06-24" @default.
• W2023250436 creator A5029713219 @default.
• W2023250436 creator A5061787764 @default.
• W2023250436 creator A5073566367 @default.
• W2023250436 date "2012-01-12" @default.
• W2023250436 modified "2023-10-05" @default.
• W2023250436 title "The Drosophila melanogaster Seminal Fluid Protease “Seminase” Regulates Proteolytic and Post-Mating Reproductive Processes" @default.
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• W2023250436 doi "https://doi.org/10.1371/journal.pgen.1002435" @default.
• W2023250436 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3257295" @default.
• W2023250436 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22253601" @default.
• W2023250436 hasPublicationYear "2012" @default.
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