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• W2023251152 endingPage "409" @default.
• W2023251152 startingPage "397" @default.
• W2023251152 abstract "Polynucleotide phosphorylase (PNPase), a homotrimeric exoribonuclease present in bacteria, is involved in mRNA degradation. In Escherichia coli, expression of this enzyme is autocontrolled at the translational level. We introduced about 30 mutations in the pnp gene by site-directed mutagenesis, most of them in phylogenetically conserved residues, and determined their effects on the three catalytic activities of PNPase, phosphorolysis, polymerisation and phosphate exchange, as well as on the efficiency of translational repression. The data are presented and discussed in the light of the crystallographic structure of PNPase from Streptomyces antibioticus. The results show that both PNPase activity and the presence of the KH and S1 RNA-binding domains are required for autocontrol. Deletions of these RNA-binding domains do not abolish any of the three catalytic activities, indicating that they are contained in a domain independent of the catalytic centre. Moreover, the catalytic centre was located around the tungsten-binding site identified by crystallography. Some mutations affect the three catalytic activities differently, an observation consistent with the presence of different subsites." @default.
• W2023251152 created "2016-06-24" @default.
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• W2023251152 creator A5071289053 @default.
• W2023251152 creator A5071648660 @default.
• W2023251152 date "2002-08-01" @default.
• W2023251152 modified "2023-10-15" @default.
• W2023251152 title "Mutational Analysis of Polynucleotide Phosphorylase from Escherichia coli" @default.
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• W2023251152 doi "https://doi.org/10.1016/s0022-2836(02)00645-9" @default.
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