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- W2023254648 abstract "A mutant (erg6) of Saccharomycescerevisiae defective in S-adenosylmethionine: Δ24-sterol-C-methyl transferase (EC2.1.1.41) which normally produces cholesta-5,7,24-trienol and cholesta-5,7,22,24-tetraenol as the major sterols (total 4,4-desmethyl sterol content-8.3fg/cell) was shown to synthesize trace levels of cholesterol (0.08fg/cell). The identity of cholesterol was established by co-chromatography in TLC, GLC and HPLC with an authentic sample, mass spectroscopy and after an incubation with [1-14C]acetate by isotopic dilution and recrystallization of the radiochemically purified material to constant specific activity." @default.
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- W2023254648 date "1988-08-01" @default.
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- W2023254648 title "Biosynthesis of cholesterol in the yeast mutant ERG6" @default.
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- W2023254648 doi "https://doi.org/10.1016/s0006-291x(88)81116-1" @default.
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