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• W2023256743 startingPage "489" @default.
• W2023256743 abstract "Episomal vectors, described for efficient and regulated expression of heterologous proteins in mammalian cells, have the advantage that they persist in multiple copies in the cell without integrating into the chromosome. To efficiently express heterologous proteins we used such a vector based on elements of the Epstein-Barr virus (EBV), namely the sequences coding for Epstein-Barr nuclear antigen 1 and the viral origin of replication. Because constitutive expression is often deleterious to the cell, we combined the interferon-inducible Mx promoter with this EBV-derived vector. This resulted in an efficient and strictly regulated expression of the reporter gene chloramphenicol acetyltransferase (CAT) and of the neurotransmitter receptor h5-HT(1B), reaching levels of 16 ng CAT/mg cytoplasmic protein and 1300 fmol receptor/mg membrane protein, respectively. For both proteins, the expression levels were influenced by the orientation of the expression cassette. The higher expression in the favored orientation did not result from a higher copy number of these episomes. Northern analysis revealed a transcriptional read-through from the thymidine kinase promoter on the episomal vector, which interfered with the transcription of the heterologous gene in the less favored orientation." @default.
• W2023256743 created "2016-06-24" @default.
• W2023256743 creator A5018767436 @default.
• W2023256743 creator A5022248069 @default.
• W2023256743 creator A5053881794 @default.
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• W2023256743 date "2003-11-19" @default.
• W2023256743 modified "2023-10-15" @default.
• W2023256743 title "Orientation‐dependent gene expression with Epstein‐Barr virus‐derived vectors" @default.
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• W2023256743 doi "https://doi.org/10.1016/s0014-5793(03)01311-5" @default.
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• W2023256743 hasPublicationYear "2003" @default.
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