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• W2023266087 abstract "In this study, we performed gel mobility shift assays using tobacco BY-2 nuclei extracts to identify the plant telomere-binding proteins (TBP). Although no complexes were detected using C-strand as a probe, a single DNA-protein complex was detected using single-stranded 32P-(TTTAGGG)4 as a probe. In competition experiments, formation of the complex was inhibited only when an ssG-strand telomere repeat was used as a competitor. These results indicate that the observed band reflects a G-strand specific single-stranded telomere-binding protein (NtGTBP1). We purified the binding protein and subsequently used RT-PCR to isolate a gene encoding the protein. The sequence reveals that the protein (NtGTBP1) is a novel TBP from a higher plant, and a search for conserved domains showed that NtGTBP1 contains two RNA recognition motifs (RRMs)." @default.
• W2023266087 created "2016-06-24" @default.
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• W2023266087 creator A5081895401 @default.
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• W2023266087 date "2004-01-01" @default.
• W2023266087 modified "2023-09-27" @default.
• W2023266087 title "Characterization and gene cloning of telomere-binding protein from tobacco BY-2 cells" @default.
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• W2023266087 doi "https://doi.org/10.1016/j.plaphy.2003.10.002" @default.
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