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- W2023267785 abstract "The cytoskeleton is conventionally viewed as being composed of three filamentous networks; microfilaments, microtubules, and intermediate filaments. This view is challenged by the findings that metabolic enzymes can form filaments with structural functions. We report that phosphofructokinase-1 (PFK1), the first rate-limiting step of glycolysis, assembles into filaments in vitro and in cells. Transmission electron microscopy (TEM) showed that purified liver PFK1 is mainly tetrameric and occasionally formed short filaments in the absence of substrate. Adding the substrate fructose 6-phosphate (F6P) induced the assembly of predominantly long filaments measuring up to 250 nm. PFK1 filaments were less rigid than actin polymers, displaying right angles in contiguous assemblies. The filaments were composed of individual tetramers and had a uniform 11 nm width, resembling an organized addition of subunits forming polymers. Regulated assembly into filaments was also indicated by light scattering measurements that showed a rapid substrate-dependent increase in scattering followed by a stable plateau. Increased light scattering was blocked by excess ATP, which inhibits PFK1 activity. To further confirm activity-dependent filament assembly we generated an inactive but tetrameric liver PFK1 mutant, His199Tyr, and found that in the presence of F6P it does not form filaments, as determined by TEM, or show an increase in light scattering. To assess filament formation by PFK1 in cells, we expressed GFP-tagged PFK1 and used live-cell imaging to examine GFP-PFK1 dynamics. Confocal microscopy reveled that cytosolic PFK1 was recruited to the distal margin of lamellipodia that were devoid of mitochondria. TIRF microscopy reveled that GFP-PFK1 formed dynamic punctae. These data indicate that active but not inactive PFK1 assembles into tetramer-aligned filaments. The activity-dependent recruitment and assembly of PFK1 filaments at the plasma membrane could provide a scaffolding and structural framework for localized ATP production in lamellipodia that lack mitochondria." @default.
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- W2023267785 date "2014-01-01" @default.
- W2023267785 modified "2023-09-28" @default.
- W2023267785 title "Filament Assembly by Phosphofructokinase-1, the Gatekeeper of Glycolysis" @default.
- W2023267785 doi "https://doi.org/10.1016/j.bpj.2013.11.3777" @default.
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