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• W2023268775 abstract "Elevated hyaluronidase levels are found in the urine of bladder and prostate cancer patients. Therefore, HA-ase is regarded as an important biomarker for the detection of these cancers. In this report, we use a FRET based ratiometric sensing approach to detect the level of HA-ase in synthetic urine. For this, we have used a HA-FRET probe (hyaluronan) labeled with fluorescein as a donor and rhodamine as an acceptor. We monitor the digestion of our HA-FRET probe with different concentrations of HA-ase in synthetic urine via fluorescence emission. The extent to which FRET is released depends on the concentration of HA-ase. Our fluorescence intensity results are also supported with time resolved fluorescence decay data. This assay can be used to develop a non-invasive technique for the detection of bladder and/or prostate cancer progression. Keywords: Ratiometric sensing, Hyaluronidase, HA-FRET, Bladder cancer." @default.
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• W2023268775 date "2013-04-01" @default.
• W2023268775 modified "2023-09-28" @default.
• W2023268775 title "FRET Based Ratio-Metric Sensing of Hyaluronidase in Synthetic Urine as a Biomarker for Bladder and Prostate Cancer" @default.
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• W2023268775 doi "https://doi.org/10.2174/13892010113149990222" @default.
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