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• W2023270947 abstract "A method was developed using zonal centrifugation to recover liver mitochondria quantiatively and free of other cellular components from a sample of whole homogenate. The fractions containing mitochondria were identified by the distribution of cytochrome oxidase and these fractions contained over 90% of the total cytochrome oxidase recovered. The mitochondrial fractions were found to be only slightly contaminated by 5′-nucleotidase (plasma membranes), acid phosphatase (lysosomes), glucose-6-phosphatase (microsomes), and catalase (peroxisomes). There was no detectable contamination by nuclear DNA (nuclei). This method was used to quantitate total liver mitochondrial protein. The development of this procedure provides a means for following total changes in mitochondrial components during mitochondrial biogenesis." @default.
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• W2023270947 date "1978-03-01" @default.
• W2023270947 modified "2023-10-16" @default.
• W2023270947 title "Quantitative isolation of liver mitochondria by zonal centrifugation" @default.
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• W2023270947 doi "https://doi.org/10.1016/0003-2697(78)90297-x" @default.
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